Journal of Animal Science and Technology
Korean Society of Animal Science and Technology
Article

Diversity of MHC-B SNP haplotypes in the Vietnamese Ri chicken

Seonju Nam1, Prabuddha Manjula2, Jaewon Kim1, Minjun Kim1, Eunjin Cho3, Trisha Agulto1, Yeong Ho Hong4, Jun Heon Lee1,3,*
1Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea.
2Department of Animal Science, Uva Wellassa University, Badulla 90000, Sri Lanka.
3Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea.
4Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
*Corresponding Author: Jun Heon Lee, Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea, Republic of. Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea, Republic of. Phone: +82-42-821-7031. E-mail: junheon@cnu.ac.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 26, 2024; Revised: May 17, 2024; Accepted: May 20, 2024

Published Online: May 20, 2024

Abstract

Avian influenza (AI) is a serious global threat to poultry and public safety, although some native chicken varieties show resilience, such as the Ri chicken in Vietnam. Major histocompatibility complex B (MHC-B), a critical component of the chicken immune system, has been shown to influence disease resistance. This study examined the MHC-B haplotype diversity in a Ri chicken population that is sensitive to avian influenza. Ri chickens were genotyped for MHC-B single nucleotide polymorphisms (SNPs) using the Kompetitive Allele Specific Polymerase Chain Reaction (KASP). Statistical tests revealed no significant differences in allele frequencies of the SNPs between resistant (R) and susceptible (S) groups. Haplotype analysis identified 32 unique haplotypes, with only one shared haplotype between the R and S groups. However, a phylogenetic analysis did not find distinct clustering of MHC-B alleles of the Ri chicken groups. Further research with a larger sample size is recommended to establish representative group-specific haplotypes and enhance our comprehension of the intricate genetic mechanisms underlying disease resistance in poultry. The implications of this research extend to improving disease resistance strategies and guiding selective breeding practices in the poultry industry

Keywords: Avian influenza; Ri chicken; MHC-B variability; SNP genotyping