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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2024.e50

Article

Complete genome sequence of potential probiotic Ligilactobacillus ruminis CACC881 isolated from swine

Soyeon Park1, Mi Ae Park1, Hyun-Jun Jang1, Dae-Hyuk Kim1,2, Yangseon Kim1,*
1Department of Research and Development, Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Korea.
2Department of Molecular Biology, Department of Bioactive Material Science, Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju 54896, Korea.
*Corresponding Author: Yangseon Kim, Department of Research and Development, Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup 56212, Korea, Republic of. E-mail: yangseon@cialm.or.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Feb 27, 2024; Revised: Apr 01, 2024; Accepted: May 04, 2024

Published Online: May 05, 2024

Abstract

<italic>Ligilactobacillus ruminis</italic> is a gram-positive anaerobic bacterium<italic> </italic>in the host intestinal tract. <italic>L. ruminis </italic>has not been extensively studied, resulting in limited data regarding its potential probiotic properties and genomic information. In this study, the genome of <italic>L. ruminis </italic>CACC881 was comprehensively analyzed, resulting in the prediction of potential probiotic characteristics. Additionally, a comparative genomic analysis was conducted on the five <italic>L. ruminis</italic> strains. The genome of strain CACC881 comprised one circular chromosome 2,107,343 bp in length. Among the predicted 1,935 protein-coding genes, the genome included genes associated with potential probiotic properties, such as acid/bile salt tolerance, CRISPR-related genes, and vitamin B-group genes. Notably, the genes for bacteriocin regulation/immunity (<italic>nisK</italic> and <italic>nisI</italic>) and antioxidant activity (<italic>ahpC</italic>) were exclusively found in strain CACC881, while absent in the other four <italic>L. ruminis</italic> genomes. These findings suggest that <italic>L. ruminis </italic>CACC881 is a potential probiotic with applications for the animal industry.

Keywords: Ligilactobacillus ruminis; swine; probiotics; PacBio; genome sequence