Table 3. Primer sequences of immune response and caecal microbiota genes for real-time quantitative polymerase chain reaction analysis
| Target gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | GenBank number |
| Immune response |
| TNF-α | GGGAGTGTGAGGGGTATCCT | CTGCACCTTCTGTCTCGGTT | MH180383.1 |
| IL-1β | ACAAGCCGAACAAAGCACAC | CTCCACATCTGGCTCACGTT | KY038171.1 |
| IL-12 | ATCCACTGGACCTCAGACCA | CTCAGAGTCTCGCCTCCTCT | S82489.1 |
| INF-y | TCCCAGAAGCTATCTGAGCAT | CCACCGTCAGCTACATCTGAAT | NM_205149.2 |
| sIgA | TTCCTGAGTTGCCGAGTGAC | AGGGATTTCTTGCTGGGAGC | DL232588.1 |
| MUC-2 | CGGTGATGACAACGACTCCA | AAGTTTGCACAGTCGTTCGC | AF167707.1 |
| β-actin | CCTTCCTGGGTAGGTGTCG | TGGCGTAGAGGTCCTTCCTG | AJ312193.1 |
| Caecal microbiota |
| Lactobacillus spp. | CGACTGCTCTGGTTATACCGT | TGAAGAAGGGTTTCGGCTCG | DI197694.1 |
| Bifidobacteria spp. | CAGCTCGTGTCGTGAGATGT | GATCTGACGTCATCCCCACC | MW750419.1 |
| Bacteroides spp. | TAGAGATAAGGCCCTTTGGGGT | CGAATCGGAGATTATTTAGGTGC | MZ172908.1 |
| Clostridium spp. | GTTTACGGCGTGGACTACCA | TGGAAGTCTAGAGTGCGGGA | DI335788.1 |
| Escherichia coli | CATGCCGCGTGTATGAAGA | GGGTAACGTCAATGAGCAAAG | 5NDI_C |
TNF-α, tumor necrosis factor-alpha; IL, interleukin; INF-y, interferon-gamma; sIgA, secretory IgA; MUC-2, mucin 2.