Table 6. Effect freezing and thawing treatment in addition ascorbic acids on sperm quality
Parameter | Motilty | Viability | Abnormality | PIM |
Ascorbic acid | | | | |
BF (n=35) | 70.46 ± 3.79a1) | 76.40 ± 2.79a | 19.77 ± 2.06b | 70.71 ± 3.09a |
AF (n=35) | 53.26 ± 3.04b | 60.51 ± 2.79b | 26.37 ± 2.40a | 60.68 ± 2.78b |
Δ BF–AF | 17.02 ± 0.82 | 15.89 ± 0.67 | −6.60 ± 0.53 | 10.03 ± 0.70 |
Lycopene | | | | |
BF (n=35) | 68.09 ± 3.84a | 74.14 ± 2.66a | 18.63 ± 2.03b | 68.77 ± 3.25a |
AF (n=35) | 51.85 ± 4.83b | 58.31 ± 2.62b | 24.37 ± 2.40a | 59.09 ± 2.64b |
Δ BF–AF | 16.23 ± 1.0 | 15.83 ± 0.63 | −5.74 ± 0.53 | 9.69 ± 0.71 |
Data show all mean ± standard error of means (n = 7)
Means in a column with different superscripts differ significantly at p < 0.05.
PMI, plasma membrane integrity; BF, data before freezing treatment; AF, data after thawing treatment.