Table 2. Effect of various ascorbic acid level on sperm quality before freezing

Treatment¹⁾	Sperm motility	Sperm viability	Sperm abnormality	PMI
C0 (0%)	67.42 ± 2.99^a2)	73.86 ± 2.04^ab	20.57 ± 2.37^b	66.57 ± 1.39^a
C1 (1%)	68.29 ± 1.79^ab	75.71 ± 2.14^bc	18.57 ± 2.23^ab	69.43 ± 1.61^b
C2 (2%)	70.29 ± 3.15^b	76.00 ± 2.45^bc	19.71 ± 1.79^ab	70.86 ± 1.68^c
C3 (3%)	74.14 ± 4.34^d	79.29 ± 2.75^c	19.43 ± 1.81^ab	74.29 ± 1.60^d
C4 (4%)	72.14 ± 2.27^cd	77.14 ± 1.77^bc	20.57 ± 1.61^b	72.43 ± 1.99^cd

C0 without addition of ascorbic acids; C1, with addition of 1 mg ascorbic acid into 100 mL extender; C2, with addition of 2 mg ascorbic acid into 100 mL extender; C3, with addition of 3 mg ascorbic acid into 100 mL extender, C4 with addition of 4 mg ascorbic acid into 100 mL extender.
Data show all mean ± standard error of means (n = 7).
Means in a column with different superscripts differ significantly at p < 0.05.
PMI, plasma membrane integrity.