Table 2. The conditions maintained in the amino acid analyzer for studying the changes in free amino acid composition

Parameter	Condition
Column	Hitachi HPLC packed ion exchange column (#2622PF)
Mobile phase	L-8900 buffer solution PF-1, 2, 3, 4, RG
Flow rate	Buffer solution: 0.35 mL/minNinhydrin solution: 0.30 mL/min
Detection wavelength	440 nm, 570 nm
Temperatures	Reaction coil: 135°CColumn: 30°C–70°C
Injection volume	20 μL