Table 1. The efficiency of embryo fused by SCNT using in vitro or in vivo matured oocytes
Group1) | No. of oocyte donor dogs | No. of oocytes | No. of embryos | Fusion rate (%)2) |
Injected | Fused |
In vivo | 15 | 164 | 116 | 85 | 73.28 |
In vitro | 2 | 20 | 17 | 14 | 82.35 |
In vivo, nuclear matured oocytes with 1st polar body retrieved from oocyte donor dog; In vitro, in vitro mature oocytes obtained by culturing immature oocytes without 1st polar body retrieved from oocyte donor dogs in IVM medium supplemented with hormone combination for 24 h.
Percentage calculated from number of injected oocytes.
SCNT, somatic cell nuclear transfer.