Table 4. Differentially abundant predicted microbial enzyme commission (EC) numbers (at least ≥ 0.1% average relative abundance across all samples) by inoculum effect at 24 h of incubation1)

Enzymes EC number Relative abundance (%) SEM Coefficient p-value q-value
HWG JBG
Site-specific DNA-methyltransferase (adenine-specific) EC:2.1.1.72 0.576 0.496 0.0138 −0.0238 0.0003 0.0464
Beta-ketoacyl-[acyl-carrier-protein] synthase II EC:2.3.1.179 0.222 0.192 0.0035 −0.0138 < 0.0001 0.0189
Uridine kinase EC:2.7.1.48 0.164 0.182 0.0033 0.0088 0.0003 0.0497
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase EC:2.7.7.60 0.215 0.191 0.0042 −0.0101 0.0002 0.0416
Alpha-amylase EC:3.2.1.1 0.149 0.087 0.0082 −0.0202 < 0.0001 0.0228
Phosphoribosyl-ATP diphosphatase EC:3.6.1.31 0.174 0.157 0.0026 −0.0072 0.0001 0.0324
Tryptophan synthase EC:4.2.1.20 0.346 0.318 0.0043 −0.0157 < 0.0001 0.0176
Statistical analysis was performed using MaAsLin2 with q = 0.05 as the cutoff.
HWG, Hanwoo inoculum-treated group; JBG, Jeju Black inoculum-treated group; ATP, adenosine triphosphate.