Table 2. Ruminal fermentation characteristics at 3 h and 24 h of incubation

Measurements Inoculum Diet SEM p-values
HWG JBG Growing Early fattening Late fattening Inoculum Diet Inoculum × Diet
3 h of incubation
 pH 6.84b 6.86a 6.84 6.86 6.86 0.01 0.0238 0.2480 0.1561
 Gas (mL) 16.44a 12.44b 15.50a 14.00ab 13.83b 0.57 < 0.0001 0.0372 0.3080
 DMD 35.13a 34.19b 34.83a 33.37b 35.78a 0.34 0.0127 0.0002 0.0042
 NH3-N (mg/dL) 0.54b 0.82a 0.50 0.71 0.82 0.07 0.0147 0.0636 0.1058
 Total VFA (mM) 29.28a 24.19b 26.36b 25.63b 28.23a 0.73 < 0.0001 0.0054 0.0712
 Acetate (%) 47.69a 44.82b 46.64 45.32 46.80 0.45 0.0002 0.0785 0.5411
 Propionate (%) 21.45 22.28 19.85 22.68 23.06 0.97 0.6771 0.3706 0.3288
 A:P ratio 2.81 2.01 3.20 2.00 2.03 0.39 0.3325 0.3938 0.3844
 Isobutyrate (%) 4.57 3.64 5.65 3.47 3.19 0.76 0.5590 0.3888 0.3711
 Butyrate (%) 11.92 12.96 11.79 12.93 12.62 0.38 0.1905 0.4597 0.3718
 Isovalerate (%) 5.46 5.83 6.31 5.53 5.10 0.33 0.5758 0.3371 0.3278
 Valerate (%) 8.91b 10.46a 9.75ab 10.07a 9.23b 0.23 < 0.0001 0.0124 0.1702
24 h of incubation
 pH 6.64b 6.67a 6.67 6.65 6.65 0.01 0.0257 0.2149 0.1184
 Gas (mL) 74.83a 66.56b 69.75 71.25 71.08 1.16 < 0.0001 0.5770 0.4477
 DMD 59.34a 55.74b 56.84 56.93 58.85 0.60 0.0010 0.1229 0.9205
 NH3-N (mg/dL) 0.60a 0.37b 0.38b 0.67a 0.41b 0.06 0.0273 0.0449 0.3565
 Total VFA (mM) 48.15 50.29 46.83 49.50 51.33 0.98 0.2863 0.1995 0.6846
 Acetate (%) 50.69 50.85 50.97 51.02 50.32 0.21 0.6994 0.3361 0.2701
 Propionate (%) 26.53 26.84 25.87b 26.32b 27.88a 0.25 0.3286 0.0004 0.3993
 A:P ratio 1.91 1.90 1.97a 1.94ab 1.81b 0.02 0.6432 0.0017 0.1851
 Isobutyrate (%) 2.12 2.06 2.18 2.08 2.01 0.04 0.4049 0.1952 0.6494
 Butyrate (%) 11.16 11.14 11.30a 11.32a 10.83b 0.08 0.9120 0.0099 0.7247
 Isovalerate (%) 3.44 3.37 3.52 3.40 3.29 0.05 0.5602 0.2572 0.6365
 Valerate (%) 6.07 5.73 6.16 5.86 5.67 0.11 0.1446 0.2119 0.5916
Superscripts indicate statistically significant differences (p < 0.05).
HWG, Hanwoo inoculum-treated group; JBG, Jeju Black inoculum-treated group; DMD, dry matter digestibility; VFA, volatile fatty acids; A, acetate; P, propionate.