Table 2. Oligonucleotide primers in qRT-PCR

Gene name	Sequence (forward, reverse)
GAPDH	5’-CTACTCACGGACACTTCAAG-3’
	5’-GACTACGGGGGTACAAACA-3’
IL-2	5’-CCAACTGAGACCCAGGAGTG-3’
	5’-TCTTCAGAGTAACCACTTCTCCC-3’
IL-4	5’-GTGCCCACGCTGTGCTTAC-3’
	5’-AGGAAACCTCTCCCTGGATGTC-3’
IL-6	5’-AAATCCCTCCTCGCCAATCT-3’
	5’-CCCTCACGGTCTTCTCCATAAA-3’
TNF-α	5’- GCCCTTCCTCGTAACCAGATG -3’
	5’- ACACGACAGCCAAGTCAACG-3’
IFN-γ	5’- AGCTCCCGATGAACGAC -3’
	5’- CAGGAGGTCATAAGATGCCA-3’
TGF-β	5’- ATCCTCAGCAAACTGCGTCT -3’
	5’- GCTCTTTGGCCCAATACTCA-3’
Total bacteria	5’-GCAGGCCTAACACATGCAAGTC-3’
	5’-TGCTGCCTCCCGTAGGAGT-3’
Lactobacillus spp.	5’-GCAGCAGTAGGGAATCTTCCA-3’
	5’-GCATTYCACCGCTACACATG-3’
Coliform spp.	5’-ATGGCTGTCGTCAGCTCGT-3’
	5’-CCTACTTCTTTTGCAACCCACTC-3’
Bacteroidetes spp.	5’-AACGCTAGCTACAGGCTTAACA-3’
	5’-ACGCTACTTGGCTGGTTCA-3’

qRT-PCR, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; TNF, tumor necrosis factor; IFN, interferon; TGF, transforming growth factor.