Table 1. Ingredients and calculated chemical compositions of experimental and commercial diets

Items Crude protein concentrations Commercial dog food1)
20% 30% 40%
Ingredient composition (%)
 Rice flour 43.91 37.04 30.04 -
 Chicken breast powder 6.60 14.00 21.60 -
 Egg yolk powder 8.00 8.00 7.50 -
 Lard 1.50 1.30 1.40 -
 Seaweed (Enteromorpha) 1.00 1.00 1.00 -
 Cabbage powder 1.00 1.00 1.00 -
 Calcium phosphate 1.00 0.80 0.60 -
 Calcium carbonate 0.70 0.80 0.89 -
 Potassium citrate 0.51 0.35 0.30 -
 Vitamin–mineral premix2) 0.40 0.40 0.40 -
 Salt 0.33 0.31 0.27 -
 Tryptophan 0.05 0.00 0.00 -
 Water 35.00 35.00 35.00 -
Chemical composition (% [DM basis, calculated])
 Dry matter 58.56 59.49 60.45 ≤ 12
 Crude protein 20.02 30.16 39.99 ≥ 26
 Ether extract 11.75 12.02 12.35 ≥ 12
 Crude fiber 0.29 0.28 0.28 ≤ 4.5
 Crude ash 2.19 2.47 2.68 ≤ 7.0
 NFE 65.75 55.07 44.70 -
 Ca 0.81 0.81 0.80 ≥ 1.0
 P 0.69 0.68 0.67 ≤ 2.0
 Ca/P ratio 1.18 1.19 1.20 -
 ME (kcal/kg) 4,001 4,005 4,014 3,732
These values were calculated as dry-based values.
All the experimental diets used chicken powder as the protein source, and each experimental diet was formulated to contain 20%, 30%, and 40% CP.
The commercial food was an extruded dry type (chicken and rice base) for adult dogs, and information on its chemical composition was the same as that provided by the manufacturer.
Provided per kilogram of experimental diets: vitamin A, 5,250 IU; vitamin D3, 375 IU; vitamin E, 37.5 mg; vitamin K, 0.078 mg; vitamin B1 (thiamine), 4.2 mg; vitamin B2 (riboflavin), 3.9 mg; vitamin B6 (pyridoxine), 3 mg; vitamin B12, 0.021 mg; D-calcium pantothenate, 9 mg; niacin, 45 mg; folic acid, 0.6 mg; biotin, 0.054 mg; taurine, 1,500 mg; FeSO4·H2O, 66 mg; MnSO4·H2O, 5.7 mg; ZnSO4·H2O, 75 mg; CuSO4·H2O, 11.25 mg; Na2SeO3, 0.27 mg; Ca(IO3)2, 1.35 mg.
CP, crude protein; DM, dry matter; EE, ether extract; NFE, nitrogen-free extract; ME, metabolizable energy (kcal/kg DM) = [(CP × 3.5) + (EE × 8.5) + (NFE × 3.5)] × 10.