Table 2. Longitudinal shifts of major genera¹⁾ from day 0 to day 28

Genus	Group2)	Day										SEM	p-values
		0	1	4	7	10	14	17	21	24	28		Linear	Quadratic
Prevotella	Control	19.757	17.552	20.935	19.746	23.073	18.702	18.706	19.472	22.539	19.090	0.439	0.6334	0.6876
	Cannulation	7.490	6.465	16.393	14.352	20.238	13.750	12.239	14.224	21.518	14.496	0.798	0.0022	0.0005
Fibrobacter	Control	0.425	0.686	0.868	0.767	1.069	1.950	0.173	0.993	1.501	0.684	0.136	0.3626	0.3225
	Cannulation	0.316	0.034	0.727	1.244	0.759	1.232	0.151	1.021	0.402	0.170	0.125	0.9056	0.0813
Saccharofermentans	Control	0.822	1.153	0.675	1.172	0.950	1.024	0.897	0.770	0.829	0.960	0.056	0.6370	0.8802
	Cannulation	2.378	3.282	1.048	1.450	1.134	0.667	1.159	0.817	1.229	0.855	0.153	0.0003	<.0001
Butyrivibrio	Control	0.587	0.834	0.810	1.414	0.901	1.391	1.146	1.178	0.736	1.735	0.077	0.0115	0.0378
	Cannulation	0.581	0.651	1.864	2.334	1.003	1.858	1.940	1.767	0.482	2.125	0.149	0.2073	0.1418
Pseudobutyrivibrio	Control	0.468	0.552	0.575	1.003	0.905	0.912	0.607	1.039	1.231	0.287	0.070	0.3684	0.0300
	Cannulation	0.217	0.180	0.455	0.509	0.489	0.450	0.771	0.972	0.322	0.120	0.057	0.3667	0.0023
Ruminococcus	Control	1.108	0.542	0.790	1.300	1.043	0.985	1.001	0.915	0.702	1.195	0.059	0.6085	0.8110
	Cannulation	0.505	0.676	2.508	1.420	1.528	0.626	1.255	1.318	1.504	1.122	0.127	0.7259	0.5700
Succiniclasticum	Control	3.716	4.384	2.827	3.336	4.216	2.991	3.521	2.808	3.314	3.097	0.147	0.0948	0.2345
	Cannulation	2.286	3.126	2.070	2.479	3.475	2.487	3.399	3.008	1.577	4.206	0.167	0.1871	0.3754
Selenomonas	Control	0.625	0.379	0.718	0.850	0.835	0.753	0.724	0.762	1.006	1.238	0.055	0.0011	0.0045
	Cannulation	0.035	0.022	0.348	0.030	0.157	0.215	0.126	0.216	0.150	0.110	0.020	0.3464	0.1492
Ruminobacter	Control	0.433	1.293	0.949	0.875	0.500	0.554	0.777	0.717	0.955	0.655	0.070	0.5825	0.7274
	Cannulation	0.347	0.221	1.318	1.208	0.753	1.154	0.365	0.449	0.039	0.095	0.142	0.1734	0.0635
Succinivibrio	Control	0.652	0.442	0.830	0.882	0.446	0.635	1.035	0.610	0.388	1.007	0.067	0.5309	0.8220
	Cannulation	0.052	0.035	1.523	0.015	0.029	0.736	0.327	0.321	0.000	0.056	0.122	0.4723	0.4950
Treponema	Control	0.832	2.110	1.043	0.908	1.163	1.217	0.642	1.865	2.190	1.558	0.118	0.1202	0.0840
	Cannulation	3.014	1.299	1.570	2.012	2.291	1.566	0.760	1.947	2.091	1.212	0.166	0.2129	0.4117

Major genera are taxa with a relative abundance of at least 0.5% of the total sequences.
Eight Hanwoo steers were divided into the control (n = 4) and cannulation (n = 4) groups.