Table 1. Experimental design of the co-culturing experiment

	Ec	AAFB	Ec + AAFB
Feed (g/culture)	0.1	0.1	0.1
SP medium (mL)	8	7	7
Washed Ent. caudatum monoculture (mL)	2	0	2
AAFB suspension (in SP, mL)	0	1	1
Simplex buffer (mL)	0	2	0
Total culture volume (mL)	10	10	10

Ec, Entodinium caudatum alone; AAFB, amino acid-fermenting bacterial consortium; Ec + AAFB, co-culture of Ec and AAFB.