Table 2. Oligonucleotide primers used in qRT-PCR analysis

Gene name Sequence (forward, reverse) Reference
Housekeeping gene
 GAPDH F: 5’- CTACACACGGACACTTCAAG -3’ NM_204305
R: 5’- GACTACGGGGGTACAAACA -3’
Carbohydrate transporters
 GLUT2 F: 5’ - AAAGAGAGTGTCCATCGGGC -3’ NM_207178
R: 5’ - GGAAGACGGAGATAACCGTGA -3’
 SGLT2 F: 5’ - TGCTGCGGACATCTCTGTTA -3’ NM_001293240
R: 5’ - TCAGAGCATTCCATTCATACCCTC -3’
Lipid transporters
 FABP1 F: 5’- ACTGGCTCCAAAGAATGACCAATG -3’ NM_204192
R: 5’- TGTCTCCGTTGAGTTCGGTCAC -3’
 CD36 F: 5’- GCGATTTGGTTAATGGCACT -3’ NM_001030731
R: 5’- TCTCCAACATCAATCGGTGA -3’
 DGAT2 F: 5’- AAAAGGGGATGCTGCCTATCT -3’ FJ868804
R: 5’- GCTTACGCAGCTCCATCTTCT -3’
 FATP4 F: 5’- AGGGATTTGTGAAACTGGCACT -3’ XM_419374.4
R: 5’- CTTTGGGATGGTGATGGGTT -3’
Protein transporters
 PepT1 F: 5’- TTTGACCAGCAGGGATCGAG -3’ NM_204365
R: 5’- CCAGCAAGGAACATGCCAAC -3’
 CAT1 F: 5’- CAAGAGGAAAACTCCAGTAATTGCA -3’ XM_417116
R: 5’- AAGTCGAAGAGGAAGGCCATAA -3’
 rBAT F: 5’- CTTCGCAACAGTGAGCTACCCATA -3’ XM_426125
R: 5’- TAAAGACGCTGTCTAACCCATCCAA -3’
 y+LAT1 F: 5’- AGGGTGTGTGGAGATGTCAG -3’ XM_418326
R: 5’- GAAGCTGTGTGTGAACGACG -3’
Tight junction proteins
 ZO-1 F: 5’- GTCTCCCTACTCTGGGGGAA -3’ XM_413773
R: 5’- GAACAGCAAATGCGTGCTCA -3’
 CLDN1 F: 5’- AGATCCAGTGCAAGGTGTACG -3’ NM_001013611.2
R: 5’- GCTGACAGACCTGCAATGATGA -3’
 OCLD F: 5’- ATGAGACCGACTACACCACG -3’ NM_205128.1
R: 5’- CCACATCCTGGTATTGAGGGC -3’
Inflammatory cytokines
 IL-1β F: 5’- TGCCTGCAGAAGAAGCCTCG -3’ NM_204524
R: 5’- GGAAGGTGACGGGCTCAAAA -3’
 TGF-β F: 5’- ATCCTCAGCAAACTGCGTCT -3’ NM_001318456.1
R: 5’- GCTCTTTGGCCCAATACTCA -3’
 IFN-γ F: 5’- AGCTCCCGATCAACGAC -3’ NM_205149.1
R: 5’- CAGGAGGTCATAAGATGCCA -3’
qRT-PC, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GLUT2, glucose transporter 2; SGLT2, sodium-glucose transporter 2; FABP1, fatty-acid binding protein 1; CD36, cluster of differentiation 36; DGAT2, diacylglycerol o-acyltransferase; FATP4, fatty-acid transport protein 4; PepT1, peptide transporter 1; CAT1, cationic amino acid transporter; rBAT, neutral and basic amino acid transport protein; y+LAT1, y + L amino acid transporter 1; ZO-1, zona occludens-1; CLDN1, claudin 1; OCLD, occludin; IL-1β, interleukin-1β; TGF-β, transforming growth factor β; IFN-γ, interferon-γ.