Table 1. Formula and chemical composition of experimental diets for molt induction

Items MD WB CZn MZn
Ingredient (%) 100.0 100.0 100.0 100.0
 Corn 54.00 17.74 58.96 53.44
 Wheat bran 38.98 70.00 38.59
 Soybean meal (45%) 2.70 8.80
 Rapeseed meal 2.10 6.80
 Corn gluten meal 0.58 1.95
 DDGS 2.99 9.67
 Tallow 1.00 0.30 1.00 1.00
 L-Lysine-HCl (25%) 0.90 0.14 0.47 0.90
 DL-Methionine (99%) 0.01 0.05
 Monocalcium phosphate 0.20 0.17 0.57 0.20
 Limestone 4.62 3.10 10.18 4.57
 Salt 0.07 0.25
 Vitamin premix1) 0.15 0.03 0.10 0.15
 Mineral premix1) 0.15 0.05 0.15 0.15
 Phytase 0.02 0.05
 ZnO - - 1.00 1.00
Calculated values2)
 CP (%) 9.75 14.90 15.20 9.55
 Ether extract (%) 3.86 3.76 3.96 4.02
 Crude fiber (%) 4.82 6.71 3.56 4.67
 Crude ash (%) 7.01 7.14 12.95 7.24
 Ca (%) 1.80 1.40 4.10 1.68
 P (%) 0.59 0.80 0.50 0.58
 Available P (%) 0.20 0.20 0.21 0.20
 Available Lys (%) 0.30 0.54 0.64 0.30
 Available Met (%) 0.15 0.22 0.30 0.15
 Available TSAA (%) 0.33 0.47 0.54 0.33
 Zinc (mg/kg) - - 8,000 8,000
 MEn (kcal/kg) 2,520 2,085 2,650 2,500
Chemical composition, analyzed
 DM (%) 88.00 88.57 89.67 88.41
 CP (%) 10.07 14.57 15.93 9.79
 Ether extract (%) 3.50 3.25 3.73 3.38
 Crude fiber (%) 4.73 5.48 3.94 4.27
 Crude ash (%) 6.65 7.46 14.41 7.72
 Ca (%) 1.79 1.84 4.23 1.83
 Total phosphorus (%) 1.79 0.75 0.59 0.57
 Zn (mg/kg) - - 8.573 8,417
Vitamin and mineral mixtures provided the following nutrients per kg of diet: vitamin A, 40,000 IU; vitamin D3, 8,000 IU; vitamin E, 10 IU; vitamin K3, 4 mg; vitamin B1, 4 mg; vitamin B2, 12 mg; vitamin B6, 6 mg; vitamin B12, 0.02 mg; pantothenic acid, 20 mg; folic acid, 2 mg; nicotinic acid, 60 mg; Mn, 25 mg; Zn, 50 mg; Fe, 60 mg; Cu, 10 mg; Co, 0.15 mg; Se, 0.10 mg.
Calculated values are based on raw materials.
MD, group received induced molting diet; WB, group received wheat bran based diet; CZn, group fed commercial layer diet with 8,000 ppm zinc; MZn, group fed induced molting diet with 8,000 ppm zinc; DDGS, distiller’s dried grains with soluble; ZnO, zinc oxide; CP, crude protein; P, phosphorus; Lys, lysine; Met, methionine; TSAA, the total sulfur amino acids; MEn, nitrogen correted metabolizable energy; DM, dry matter.