Table 3. Effects of anti-OPN antibody at different dilution proportions on cleavage at 48 h during IVF

Anti-OPN antibody	No. of oocytes	Cleavage at 48 h (%)
0	207	52.54 ± 0.90^a
1:50	186	50.48 ± 0.28^a
1:200	196	49.11 ± 0.93^a
1:500	182	48.58 ± 1.35^b
1:1,000	195	43.03 ± 2.52^c
1:2,000	201	47.63 ± 1.74^b

The experiment was replicated four times.
Percentages are expressed as the mean ± SEM.
Cell cleavage was no. of 2-cell/no. of oocytes.
Different superscript letters indicate significant differences (p < 0.05).
OPN, osteopontin; IVF, in vitro fertilization.