Table 1. PCR and RT-PCR primer sequences

Gene		Primer sequence (5’ to 3’)	Product size (bp)	Accession no.
OPN	F	ATGAGAATTGCAGTGATAGCCTTC	909	397087
	R	GTTGATCTCAGAAGACGCACTCTC
6His-OPN	F	ATGCATCATCATCATCATCATATGCTTCCAGTTAAACA	885	397087
	R	GTTGATCTCAGAAGACGCACTCTC
β-Actin	F	CTGTGGCATCCACGAAACTA	254	11461
	R	ACATCTGCTGGAAGGTGGAC

PCR, polymerase chain reaction; RT, reverse transcription; OPN, osteopontin; bp, base pair.