Table 1. Composition (g/kg, as-fed basis) of the experimental diets

Ingredients Day 1–21 Day 22–35
 Corn 48.51 60.96
 Wheat 8.40 4.12
 Wheat bran 4.10 -
 Soybean meal 48% 31.15 27.62
 Vegetable oil 3.30 3.30
 Limestone 1.20 0.95
 Mono-calcium phosphate 1.65 1.39
 Iodized alt 0.30 0.35
 Vitamin-mineral premix1) 0.30 0.30
 Lysine-HCI 0.34 0.30
 DL-Methionine 0.20 0.19
 L-Threonine 0.13 0.11
 L-Cystine 0.12 0.11
 Chromium oxide 0.30 0.30
Calculated values2)
 Metabolizable energy (kcal/kg) 3,050 3,200
 Crude protein (%) 22 20
 Ca (%) 0.90 0.80
 Available P (%) 0.40 0.40
 Lysin (%) 1.39 1.24
 Methionine (%) 0.52 0.49
 Methionine + cysteine (%) 0.88 0.82
 Threonine (%) 0.90 0.81
 Tryptophan (%) 0.25 0.22
 Valine (%) 0.97 0.89
 Arginine (%) 1.34 1.19
Calculated values (SID)
 Lysin (%) 1.25 1.12
 Methionine (%) 0.48 0.46
 Methionine + cysteine (%) 0.80 0.74
 Threonine (%) 0.78 0.71
 Tryptophan (%) 0.22 0.20
 Isoleucine (%) 0.76 0.69
 Leucine (%) 1.54 1.48
 Valine (%) 0.85 0.78
 Histidine (%) 0.50 0.45
 Arginine (%) 1.22 1.07
Provided per kilogram of diet: vitamin A, 12,000 IU; vitamin D3, 2,500 IU; vitamin E, 30 IU; vitamin K3, 3 mg; D-pantothenic acid, 15 mg; nicotinic acid, 40 mg; choline, 400 mg; and vitamin B12, 12 μg; Fe, 90 mg from iron sulfate; Cu, 8.8 mg from copper sulfate; Zn, 100 mg from zinc oxide; Mn, 54 mg from manganese oxide; I, 0.35 mg from potassium iodide; Se, 0.30 mg from sodium selenite.
The values were calculated according to the values of feedstuffs in NRC [16] and Ross 308 Broiler Nutrition Specification [14].
SID, standardized ileal digestibility.