Table 1. Experimental design and composition of TMRs in the experiment

Items Control T1 T2 T3
No. of cows 6 6 6 6
SCCF (2×107 CFU/mL) - 5 mL/head - 5 mL/head
Experimental diets TMR-1 TMR-2
Ingredient (% of DM)
 Concentrates 38.54 39.59
 Corn 7.73 7.95
 Full-fat soybeans 4.03 2.07
 DDGS 5.41 1.85
 Corn silage 11.84 12.17
 Whole crop barley silage 19.19 16.43
 Rice silage 10.91 7.47
 Alfalfa - 10.06
 Minerals and vitamins 2.35 2.41
Chemical compositions (% of DM)
 Crude protein 15.15 14.77
 Ether extract 5.04 5.75
 Crude fiber 18.20 17.79
 Crude ash 10.40 9.98
 NDF 33.09 33.14
 ADF 26.18 24.50
 Ca 1.28 1.25
 P 0.39 0.51
 NEL (Mcal/kg DM) 1.46 1.49
 RFV 192.58 195.97
 RFQ 5.31 5.40
TMR, total mixed ration; SCCF, Saccharomyces cerevisiae culture fluid (Agriculture Technology Center, Anseong, Korea); DM, dry matter; DDGS, dried distiller’s grains with solubles; NDF, neutral detergent fiber; ADF, acid detergent fiber; NEL, net energy for lactation; RFV, relative feed value; RFQ, relative feed quality