Table 1. List of primers specific for porcine cytokines and their characteristics as analyzed by real-time PCR¹⁾

Gene symbol	Cytokine (description)	Sequence (5’→3’)	Accession no.	Qiagen ID
IFNA1	IFN-α(Interferon-alpha-15)	F: CTCTCTGGGCTGCGACCTR: CTCCTTCCTCCTGAGTCTGTC	NM_001164845	PPS02420A
IFNG	IFN-γ(Interferon-gamma)	F: TTCAGCTTTGCGTGACTTTGR: GGTCCACCATTAGGTACATCTG	NM_213948	PPS00334A
IL6	IL-6(Interleukin-6)	F: CCTCTCCGGACAAAACTGAAR: TCTGCCAGTACCTCCTTGCT	NM_001252429	PPS19379A
CXCL8	IL-8(Interleukin-8)	F: GACCCCAAGGAAAAGTGGGTR: TGACCAGCACAGGAATGAGG	NM_213867	PPS00237A
IL10	IL-10(Interleukin-10)	F: CTGCCTCCCACTTTCTCTTGR: TCAAAGGGGCTCCCTAGTTT	NM_214041	PPS00445B
TNF	TNF-α(Tumor necrosis factor)	F: CCACGCTCTTCTGCCTACTGCR: GCTGTCCCTCGGCTTTGAC	NM_214022	PPS00426A

Two endogenous control genes—glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Qiagen ID: PPS00192A) and β-actin (ACTB, Qiagen ID: PPS00953A) — present on the PCR array were used for normalization.
PCR, polymerase chain reaction.