| Parameter | Setting |
|---|---|
| Field-of-view depth=Depth of sample chamber | 20 µm |
| Light adjustment | 90–105 |
| Total number of cells evaluated or number of fields | 1,500 spermatozoa or 14 fields |
| Sperm recognition area | 15–75 µm2 |
| Frame rate | 25 frames/sec |
| Points assessed for sperm motility | 11, VAP points 5 |
| Total motility | Progressive motility + local motility |
| Local motility | DSL (distance: straight) < 6.0 µm |
| Progressive motility | > 75% of STR (straightness) |