Table 1. Forward and reverse primers for real-time PCR for specific gene mRNA

Gene	Forward Primer sequence(5’-3’)	Reverse Primer sequence(5’–3’)	Product size (bp)	Gen Bank
GAPDH	ACTCTGGCAAAGTGGATGTTGTC	GCATCACCCCACTTGATGTTG	143	NM_001034034
Pax3	GGCTGCGTCTCTAAGATCCT	ATTTCCCAGCTGAACATGCC	158	NM_001206818
Pax7	TGCCCTCAGTGAGTTCGATT	CGGGTTCTGACTCCACATCT	180	XM_015460690
MYOD	CCGACGGCATGATGGACTA	CTCGCTGTAGTAAGTGCGGT	80	NM_001040478
MYOG	CAGTGAATGCAGCTCCCATAG	GCAGATGATCCCCTGGGTTG	86	NM_001111325
MYF5	CCCACCTCAAGTTGCTCTGA	CCGTGGCATATACATTTGGTACA	115	NM_174116
MRF4	TGGACCCCTTCAGCTACAGA	ATGCTTGTCCCTCCTTCCTTG	139	NM_181811
PPARγ	ATCTGCTGCAAGCCTTGGA	TGGAGCAGCTTGGCAAAGA	138	NM_181024
C/EBPα	CCAGAAGAAGGTGGAGCAACTG	TCGGGCAGCGTCTTGAAC	69	GU947654
C/EBPβ	CAACCTGGAGACGCAGCACAAG	CGGAGGAGGCGAGCAGAGG	143	NM_176788
SREBP1	GCAGCCCATTCATCAGCCAGACC	CGACACCACCAGCATCAACCACG	146	NM_001113302
GPR43	GGCTTTCCCCGTGCAGTA	ATCAGAGCAGCGATCACTCCAT	63	NM_001163784
SCD	TGCCCACCACAAGTTTTCAG	GCCAACCCACGTGAGAGAAG	80	NM_173959
CPT-1β	ACACATCTACCTGTCCGTGATCA	CCCCTGAGGATGCCATTCT	72	NM_001034349
ACSL3	ATGGGACTTGGGAGGAGCTGTG	GTCCACGGTTCAGGGCTCAAAC	139	NM_001205468
AMPKα	CACCAAGGTGTAAGGAAAGCA	ACGGGTTTACAACCTTCCATTC	127	NM_001109802
LPL	ACGATTATTGCTCAGCATGG	ACTTTGTACAGGCACAACCG	130	NM_001075120
FASN	CGCTTGCTGCTGGAGGTCAC	GGTCTCAGGGTCTCGGCTCAG	141	NM_001012669
GPAT3	CCTGACTTGCTGCCGCATCTG	CGATTGGGGACGTATGGTTGG	116	NM_001192514
PLIN1	ACCATCTCCACCCGCCTTCG	GCAGCATACTCGGCAGTGTCTC	140	NM_001083699
PLIN2	GCTCCATTCCGCCTTCAACCTG	GCCGATGCTTCTCTTCCACTCC	124	NM_173980
PLIN3	GCAGAGCGGCGTAGACTTGAC	CGTCATGGGCAGGTGGTTGTC	148	NM_001077046
CD36	TTGGTGATGAGAAGGCGGAA	ACCAACACTGAGCAAGACGA	92	XM_024990438
Leptin	AAGGAAAATGCGCTGTGGAC	CACAGCCTCCACGTAAGACA	73	NM_173928
ADI	CCATCCTGATGTCGTCTCAC	CTCCTCGGCTTTGGTTGGG	158	NM_001034055

PCR, polymerase chain reaction; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Pax3, paired box 3; Pax7, paired box 7; MYOD, myogenic differentiation antigen; MYOG, myogenin; MYF5, myofactor-5; MRF4, myofactor-4; PPARγ, peroxisome proliferator-activated receptor gamma; C/EBPα, CCAAT/enhancer-binding protein alpha; C/EBPβ, CCAAT/enhancer-binding protein beta; SREBP1, sterol-regulated binding protein 1; GPR43, G-protein coupled receptor 43; SCD, stearoyl coenzyme-A desaturation enzyme; CPT1β, carnitine palmitoyltransferase 1 beta; ACSL3, acyl-CoA synthetase long-chain 3; AMPKα, AMP-activated protein kinase alpha-1; LPL, lipoprotein lipase; FASN, fatty acid synthase; GPAT3, glycerin-3-phosphate acyltransferase 3; PLIN1, lipid droplet formation-related protein, perilipin; PLIN2, adipose differentiation-related protein (adipophilin); PLIN3, tail-interacting protein of 47 kDa (TIP47); ADI, adiponectin.