Table 1. Nucleotide sequences of real-time polymerase chain reaction (PCR) primers and conditions for pathogens causing calf diarrhea

Type	Microbial agents	PCR primers, probes and conditions	Primer sequences (5’ - 3’)				Reference
			Reverse transcription (°C/min)	Activation of DNA polymerase (°C/min)	Denaturation (°C/min)	Annealing/extension (°C/min)
Viruses (PCR type 1)	Bovine viral diarrhea virus	BVD-F	GGG NAG TCG TCA RTG GTT CG				[23]
		BVD-R	GTG CCA TGT ACA GCA GAG WTT TT
		BVD-Probe (CY5/BHQ2)	CCA YGT GGA CGA GGG CAY GC
	Bovine coronavirus	BCV-F	CTA GTA ACC AGG CTG ATG TCA ATA CC				[12]
		BCV-R	GGC GGA AAC CTA GTC GGA ATA
		BCV-Probe (FAM/MGB)	CGC CTG ACA TTC TCG ATC
	Bovine rotavirus	BRV-F	TCA ACA TGG ATG TCC TGT ATT CCT				[24]
		BRV-R	TCC CCC AGT TTG GAA TTC ATT
		BRV-Probe (VIC/MGB)	TCA AAA ACT CTT AAA GAT GCA AG
	Conditions		45/10	95/10	95/0.25	60/1
Bacteria/parasites (PCR type 2)	Escherichia coli K99	K99-F	GCT ATT AGT GGT CAT GGC ACT GTA G				[25]
		K99-R	TTT GTT TTC GCT AGG CAG TCA TTA
		K99-Probe (FAM/BHQ1)	ATT TTA AAC TAA AAC CAG CGC CCG GCA
	Cryptosporidium parvum	Cryptosporidium parvum-F	CAA ATT GAT ACC GTT TGT CCT TCT GT				[26]
		Cryptosporidium parvum-R	GGC ATG TCG ATT CTA ATT CAG CT
		Cryptosporidium parvum-Probe (JOE/BHQ1)	TGC CAT ACA TTG TTG TCC TGA CAA ATT GAA
	Salmonella species	Salmonella-F	GGG NAG TCG TCA RTG GTT CG				[27]
		Salmonella-R	GTG CCA TGT ACA GCA GAG WTT TT
		Salmonella-Probe (CY5/BHQ2)	CCA YGT GGA CGA GGG CAY GC
	Conditions		N/A	95/10	95/0.25	60/1