Table 6. Effect of harvest and wilting time on microbial counts of rye silage

Harvest date Wilting period1) LAB F&Y TM
Log CFU/g of FM
April 30 (Boot) 0 h 3.85b 3.95c 4.75b
6 h 6.02a 5.13b 6.06a
24 h 6.03a 5.49a 6.07a
Mean 5.30C 4.86B 5.63C
May 9 (Heading) 0 h 6.44c 5.00a 7.09b
6 h 6.83b 2.92b 6.98b
24 h 7.34a 3.16b 7.92a
Mean 6.87A 3.70C 7.33A
May 15 (Blooming) 0 h 5.97c 5.26c 6.02c
6 h 6.52b 5.95b 6.58b
24 h 7.37a 7.50a 7.62a
Mean 6.62B 6.24A 6.74B
SEM 0.08 1.04 2.36
p-value H < .0001 < .0001 < .0001
W < .0001 < .0001 0.6022
H × W < .0001 < .0001 0.0004
0 h, no wilting before ensiling; 6 h, wilted for 6 hours before ensiling; 24 h, wilted for 24 hours before ensiling.
Within a column, different superscripts in capital letters indicate that main plots differ;
those in lower-case letters indicate that sub-plots differ (p < 0.05).
LAB, lactic acid bacteria; F&Y, fungi and yeast; TM, total microorganisms; CFU, colony forming units; FM, fresh matter; H, Harvest date; W, wilting.