| Variable | Condition |
|---|---|
| Column | CAPCELL PAK C18 UG120 S5, 4.6 mm I. D. × 250 mm |
| Mobile phase | A: acetonitrile: methanol (85: 15) |
| B: dichloromethanol | |
| A (70%), B (30%) | |
| Flow rate | 1.0 mL / min |
| Injection volumn | 3.5 μL |
| Temperature | 35°C |
| Pressure | 4.9 MPa |
| Detector | UV 254 nm |