Table 5. Comparison of fusion and embryonic developmental rates of oocytes produced using conventional SCNT or modified HMC
Group | No. of oocytes (%, mean ± SEM) | No. of embryos developed (%, mean ± SEM) |
Injected | Fused | 8 cell (%) | 16 cell (%) | Blastocyst (%) |
SCNT | 322 | 184 (57.1 ± 5.8)a | 148 (80.4 ± 2.31)a | 101 (54.9 ± 5.00)a | 25 (13.6 ± 1.99) |
HMC | 240 | 207 (86.3 ± 2.4)b | 144 (69.6 ± 5.37)b | 89 (43.0 ± 3.75)b | 37 (17.9 ± 1.44) |
Proportions calculated on the number of oocytes in each group.
The experiments were repeated seven times.
Values with different letters in the same column are significantly different at p < 0.05.
SCNT, somatic cell nuclear transfer; HMC, handmade cloning.