Table 3. Incidence of oocytes with cytoplasmic protrusions according to demecolcine treatment in oocyte status

Oocyte status	No. of oocytes examined	No. of oocytes with cytoplasmic protrusions (%, mean ± SEM)1)
After IVM	114	90 (78.89 ± 1.84)a
Denuded oocyte	131	60 (45.75 ± 0.54)b
ZP removed oocyte	125	64 (51.09 ± 1.77)b

After IVM, 44 h after oocyte maturation, cumulus cells were removed.; Denuded oocytes, those immediately after removing the cumulus cells; ZP removed oocyte, those immediately after removing the zona pellucida.
Percentage per oocyte examined. The experiments were repeated three times.
Values with different letters in the same column are significantly different at p < 0.05.
IVM, in vitro maturation; ZP, zona pellucida.