Table 2. Incidence of oocytes with cytoplasmic protrusions according to demecolcine treatment time

Treatment time (min)	No. of oocytes examined	No. of oocytes with cytoplasmic protrusions (%, mean ± SEM)1)
30	125	65 (51.84 ± 3.80)a
60	127	86 (67.64 ± 2.15)ab
90	114	90 (78.89 ± 1.84)b
120	122	84 (68.79 ± 3.49)ab

Percentage per oocyte examined. The experiments were repeated three times.
Values with different letters in the same column are significantly different at p < 0.05.