Percentage motility (%) | 93.30 | 90.43 | 90.38 | 89.79 | 90.40 | 90.11 | 92.18 |
Progressive motility (%) | 55.05d | 69.64c | 83.68a | 81.08ab | 84.55a | 83.55a | 72.57bc |
Non- progressive motility (%) | 38.25a | 20.80b | 6.70c | 8.71c | 5.85c | 6.57c | 19.61b |
Curvilinear velocity (VCL) (μm/s) | 15.00b | 21.45b | 27.29a | 28.94a | 28.32a | 28.22a | 22.43ab |
Average path velocity (VAP) (μm/s) | 5.74b | 19.45a | 19.40a | 20.25a | 19.70a | 19.72a | 22.46a |
Straight line velocity (VSL) (μm/s) | 7.30b | 9.72a | 8.04ab | 8.30a | 8.02ab | 8.09ab | 9.48a |
Linearity (%) | 30.18b | 52.90a | 53.30a | 52.71a | 51.95a | 52.44a | 54.40a |
Straightness (%) | 46.38b | 61.84a | 62.89a | 61.96a | 61.46a | 61.87a | 63.93a |
Amplitude of lateral head (ALH) (μm) | 0.76a | 0.42c | 0.50c | 0.50c | 0.48c | 0.49c | 0.62b |
Beat cross frequency (BCF) (Hz) | 4.48a | 3.77b | 4.35a | 4.70a | 4.68a | 4.58a | 5.00a |
Wobble (%) | 64.44b | 70.78a | 54.33b | 54.13b | 52.99b | 53.57b | 55.54b |
Viability (%) | 93.30 | 90.43 | 90.38 | 89.79 | 90.40 | 90.11 | 92.18 |