Table 3. Effect of a multi-protease in the diet on growth performance of broiler chickens^1),2)

	PC	PC-150	NC	NC-150	NC-300	ENC	ENC-150	ENC-300	SEM	p-value⁴⁾
Crude protein (%)	-	-	−0.5	−0.5	−0.5	−1	−1	−1
Amino acid³⁾ (%)	-	-	−2	−2	−2	−4	−4	−4
Day 7
BW (g)	177.26^ab	180.38^b	171.52^ab	174.51^ab	179.56^a	168.59^b	170.30^a	174.49^ab	1.073	0.038
ADFI (g/bird)	23.40	23.34	26.30	24.03	23.42	22.92	24.66	24.17	0.546	0.867
ADG (g/bird)	19.62^ab	20.08^a	18.81^ab	19.20^ab	19.96^a	18.35^b	18.61^b	19.23^ab	0.154	0.032
FCR (g feed/g gain)	1.19	1.16	1.39	1.26	1.17	1.25	1.33	1.26	0.027	0.387
Day 14
BW (g)	440.81	447.71	431.39	434.76	446.74	428.26	429.27	433.87	2.065	0.099
ADFI (g/bird)	49.36	47.12	54.24	48.46	48.83	49.82	52.72	49.20	0.948	0.641
ADG (g/bird)	37.65	38.19	37.12	37.18	38.17	37.10	37.00	37.05	0.318	0.954
FCR (g feed/g gain)	1.32	1.24	1.46	1.31	1.29	1.34	1.43	1.34	0.018	0.061
Day 21
BW (g)	872.90	877.47	865.42	868.59	876.84	857.43	863.96	871.57	4.266	0.950
ADFI (g/bird)	89.93	85.66	81.49	90.16	86.3	88.91	86.16	83.85	1.112	0.504
ADG (g/bird)	61.73	61.39	62.00	61.98	61.44	61.31	62.1	62.53	0.557	0.999
FCR (g feed/g gain)	1.47	1.41	1.32	1.46	1.42	1.45	1.39	1.36	0.016	0.302
Day 28
BW (g)	1,446.29^ab	1,455.18^a	1,416.61^ab	1,426.38^a	1,448.13^a	1,359.90^b	1,410.83^ab	1,435.59^a	7.123	0.020
ADFI (g/bird)	122.32	115.41	125.26	116.15	115.32	122.68	118.17	120.80	1.672	0.747
ADG (g/bird)	81.91^a	82.53^a	78.74^a	79.68^a	81.61^a	71.78^b	78.13^ab	80.57^a	0.832	0.032
FCR (g feed/g gain)	1.52^bc	1.40^b	1.61^ab	1.46^b	1.44^b	1.71^a	1.52^bc	1.50^bc	0.018	< 0.001
Day 35
BW (g)	2,188.27^a	2,222.38^a	2,131.93^abc	2,157.62^abc	2,188.90^a	2,062.95^c	2,083.53^bc	2,166.81^ab	12.046	0.009
ADFI (g/bird)	175.89	172.84	162.39	173.92	179.16	175.39	177.95	174.70	2.573	0.856
ADG (g/bird)	106.00	109.60	102.19	104.46	105.82	100.44	96.10	104.46	1.121	0.110
FCR (g feed/g gain)	1.68	1.58	1.58	1.71	1.72	1.77	1.89	1.69	0.029	0.203
Day 1–21
ADFI (g/bird)	54.23	52.04	54.01	54.22	52.85	53.88	54.51	52.41	0.609	0.962
ADG (g/bird)	39.67	39.89	39.31	39.45	39.86	38.92	39.23	39.60	0.203	0.945
FCR (g feed/g gain)	1.33	1.27	1.39	1.34	1.29	1.35	1.38	1.32	0.012	0.172
Day 22–35
ADFI (g/bird)	149.11	144.12	143.83	145.04	147.24	149.03	148.06	147.75	1.839	0.993
ADG (g/bird)	93.95^a	96.06^a	90.47^abc	92.07^abc	93.72^a	86.11^c	87.11^bc	92.52^ab	0.743	0.006
FCR (g feed/g gain)	1.60^ab	1.49^b	1.61^ab	1.46^ab	1.44^ab	1.71^a	1.52^a	1.50^ab	0.019	0.033
Day 1–35
ADFI (g/bird)	92.18	88.87	89.93	90.55	90.61	91.94	91.93	90.54	0.946	0.991
ADG (g/bird)	61.38^a	62.36^a	59.77^abc	60.50^abc	61.40^a	57.80^c	58.38^bc	60.77^ab	0.344	0.009
FCR (g feed/g gain)	1.43^ab	1.36^b	1.48^a	1.44^ab	1.41^ab	1.51^a	1.51^a	1.43^ab	0.012	0.030

The eight dietary treatments were: positive control (PC), negative control (NC: minus 0.5% from PC, and minus 2% of lysine, methionine, threonine and methionine plus cysteine), extreme negative control (ENC: minus 1% from PC, minus 4% of lysine, methionine, threonine and methionine plus cysteine), and plus multi-protease 150 or 300 g per ton (e. g., PC-150); PC, PC-150, NC, NC-150, NC-300, ENC, ENC-150, ENC-300.
Values are the mean of eight replicates per treatment.
List of amino acids: lysine, methionine, threonine and methionine + cystine.
There are no significance linear-plateau and quadratic-plateau regression obtain on growth performance for 5 weeks after hatching.
Means with different superscripts in the same row were significantly different (Turkey’s multiple range test, p < 0.05).
BW, body weight; ADFI, average daily feed intake; ADG, average daily gain; FCR, feed conversion ratio.