Table 2. Analyzed chemical composition of the experimental diets1)
| PC_1-3 | PC_4-5 | NC_1-3 | NC_4-5 | ENC_1-3 | ENC_4-5 |
Analyzed composition |
Gross energy (kcal/kg) | 3,460 | 3,600 | 3,460 | 3,600 | 3,460 | 3,600 |
Crude protein (%) | 21.52 | 19.52 | 21.03 | 19.02 | 20.55 | 18.52 |
Total amino acid (%) |
Lysine | 1.34 | 1.20 | 1.32 | 1.17 | 1.29 | 1.15 |
Methionine | 0.53 | 0.48 | 0.51 | 0.47 | 0.50 | 0.45 |
Cystine | 0.48 | 0.44 | 0.47 | 0.43 | 0.46 | 0.43 |
Threonine | 0.95 | 0.85 | 0.92 | 0.84 | 0.89 | 0.81 |
Tryptophan | 0.24 | 0.22 | 0.24 | 0.21 | 0.23 | 0.20 |
Iso-Leucine | 0.87 | 0.78 | 0.84 | 0.76 | 0.82 | 0.73 |
Leucine | 1.02 | 0.93 | 1.00 | 0.90 | 0.97 | 0.88 |
Valine | 0.98 | 0.89 | 0.95 | 0.86 | 0.93 | 0.84 |
Histidine | 0.61 | 0.56 | 0.59 | 0.54 | 0.58 | 0.52 |
Arginine | 1.41 | 1.26 | 1.37 | 1.22 | 1.32 | 1.18 |
Methionine + cystine | 1.00 | 0.93 | 0.98 | 0.90 | 0.96 | 0.88 |
The eight dietary treatments were: positive control (PC), negative control (NC: minus 0.5% from PC, and minus 2% of lysine, methionine, threonine and methionine plus cysteine), extreme negative control (ENC: minus 1% from PC, minus 4% of lysine, methionine, threonine and methionine plus cysteine), and plus multi-protease 150 or 300 g per ton (e. g., PC-150); PC, PC-150, NC, NC-150, NC-300, ENC, ENC-150, ENC-300.