Table 3. Gas production after in vitro incubation of treatments using buffered rumen fluid

Item	Treatment				SEM	p-value
Item	RGP	CGF	WG	CGM	SEM	p-value
Total gas (mL/g DM)
3 h	10.7^b	14.2^a	13.5^a	9.0^c	0.41	< 0.01
6 h	34.5^a	28.6^b	33.8^a	27.4^b	0.56	< 0.01
12 h	78.4^a	55.6^c	75.3^ab	72.2^b	1.91	< 0.01
24 h	152.0^a	118.9^b	145.0^a	152.7^a	3.44	< 0.01
36 h	199.2^a	170.4^c	187.2^b	200.7^a	2.79	< 0.01
48 h	224.9^a	207.8^b	211.9^b	227.2^a	2.48	< 0.01
CO₂ (mL/g DM)
12 h	62.54^a	48.02^b	65.25^a	61.25^a	3.592	< 0.01
24 h	126.81^a	98.94^b	121.25^a	128.25^a	5.783	< 0.01
36 h	156.06^a	130.64^b	145.62^a	153.08^a	4.022	< 0.01
48 h	171.59^a	152.48^b	160.69^ab	171.58^a	4.448	< 0.01
CO₂ ratio¹⁾	76.28	73.36	75.83	76.35	2.033	0.45
CH₄ (mL/g DM)
12 h	4.02^a	2.5^b	4.04^a	3.34^a	0.247	< 0.01
24 h	8.82^a	6.62^b	9.18^a	8.62^a	0.483	< 0.01
36 h	11.83^a	10.02^b	11.71^a	10.89^ab	0.382	< 0.01
48 h	13.40^a	12.26^ab	13.13^ab	12.11^b	0.439	< 0.05
CH₄ ratio¹⁾	5.95^b	5.90^b	6.19^a	5.53^b	0.156	< 0.05

Values in the same row with different letters differ significantly (p < 0.05).
CO₂ and CH₄ ratio as portion of total gas produced.
RGP, red ginseng byproduct; CGF, corn gluten feed; WG, wheat gluten; CGM, corn germ meal.