Table 3. Primers used for quantitative PCR

Target species	Primer	Sequence (5’ → 3’)	Size(bp)	Efficiency1)	References
General bacteria	F	CGGCAACGAGCGCAACCC	130	1.89	[11]
	R	CCATTGTAGCACGTGTGTAGCC
Protozoa	F	GCTTTCGWTGGTAGTGTATT	223	1.92	[14]
	R	CTTGCCCTCYAATCGTWCT
Fungi	F	GAGGAAGTAAAAGTCGTAACAAGGTTTC	120	2.06	[11]
	R	CAAATTCACAAAGGGTAGGATGATT

Efficiency is calculated as [10^−1/slope].
PCR, polymerase chain reaction; bp, base pair.