Table 1. Alpha diversity statistics among the three groups (three periods of exposure to short-term heat stress) of Hanwoo steers (n = 4)

Domain	Sample group (n = 4)	Sampling type¹⁾	No. of sequences²⁾	No. of OTUs	Chao1	Shannon diversity index	PD_whole_tree
Bacteria	0 day	Subsampled reads	40,000	4,441^a	16,647^a	6.602^a	210^a
	3 day	Subsampled reads	40,000	4,618^a	15,802^a	6.644^a	217^a
	6 day	Subsampled reads	40,000	4,198^a	14,289^a	6.270^a	199^a
Archaea	0 day	Subsampled reads	40,000	1,310^a	4,169^a	3.378^a	20^a
	3 day	Subsampled reads	40,000	1,285^a	3,773^a	3.426^a	21^a
	6 day	Subsampled reads	40,000	1,239^a	3,884^a	2.984^a	20^a

Means of the three groups were compared using ANOVA followed by Duncan’s test.
From each rumen sample, 40,000 sequences were randomly subsampled to normalize the number of OTUs.
Means with the same superscript letter within a column indicate non-significant differences (p > 0.05).
OTUs, operational taxonomy units; PD, phylogenetic diversity.