Table 1. Oligonucleotide primer sequences used for qPCR amplification

Gene symbol	5’-3’ sequence	Annealing temp (°C)	qPCR amplicon size (bp)	GC	Efficiency of qPCR reaction (%)
ALOX2	F: AATCACAGGCCGGTGTGAAC	60.89	80	55.00	94
	R: AGTCCTCCTTTACTGGCTCTG	58.82		52.38
TDO2B	F: GTTCTGCAGAGCTTGAGGGT	60.00	95	55.00	100
	R: TCTTGCTCGGACTTCAGCAG
SYT4	F: CTTCTGACCTGGAGAGCGTG	60.11	72	60.00	92
	R: AGCTATCGGGGGAAACTACCT	60.06		52.38
CSN2	F: CCAGCCTCTTCCTCCAACTG	60.04	75	60.00	91
	R: ACAGGCAGGACTTTGGACTG	59.89		55.00
RPS23	F: GGAGTTGAAGCCAAACAGCC	59.00	90	59.68	90
	R: TGGGAACAAAAGCAGTGATCT	58.86		57.77
CES1	F: CGGAGGAGGTCTCATGGTG	59.19	97	63.16	100
	R: CAGGCGGTACTGAATGGTCA	59.75		55.00

qPCR, quantitative PCR.