Table 7. Equations for linear regression of ruminal fermentation parameters on 3-nitrooxypropanol levels (mg/kg of DMI) from dairy database

Parameter	Parameter estimates							Model statistics
	N	intercept	SE intercept	p-value	Slope	SE slope	p-value	RMSE	R2
Total VFA (mM)	10	99.762	4.0520	0.0001	−0.0240	0.02516	0.4102	3.404	0.051
Acetate (%)	10	59.653	2.0003	< 0.0001	−0.0339	0.00851	0.0284	1.070	0.769
Propionate (%)	10	21.831	0.9936	0.0002	0.0124	0.00662	0.1591	0.851	0.466
Butyrate (%)	9	13.855	0.2088	< 0.0001	0.0068	0.00588	0.3667	0.680	0.528
Iso-butyrate (%)	10	0.922	0.1383	0.0069	0.0002	0.00021	0.3832	0.051	0.000
Valerate (%)	10	1.952	0.1569	0.0011	0.0025	0.00068	0.0340	0.115	0.522
Iso-valerate (%)	10	1.456	0.3296	0.0215	0.0049	0.00335	0.2395	0.304	0.160
A:P ratio	10	2.811	0.2202	0.001	−0.0028	0.00098	0.0628	0.157	0.552
DMI (g/kg)	17	22.051	1.4831	< 0.0001	−0.0032	0.00202	0.1760	0.316	0.211
Milk yield (kg/d)	17	32.557	2.9927	0.0001	−0.0122	0.00507	0.0606	0.791	0.381
Milk fat (%)	17	3.845	0.1514	< 0.0001	0.0012	0.00058	0.0861	0.092	0.321
Milk protein (%)	17	3.275	0.0873	< 0.0001	0.0005	0.00023	0.0838	0.033	0.322
Milk lactose (%)	14	4.597	0.0897	< 0.0001	0.0001	0.00010	0.2263	0.015	0.214
FCM (kg/d)	14	31.771	3.7451	0.0011	0.0072	0.01169	0.5718	1.109	0.008

VFA, volatile fatty acids; A:P ratio, acetate to propionate ratio; DMI, dry matter intake; FCM, 4% fat corrected milk; SE, standard error; RMSE, residual mean square error; R², coefficient of determination.