Table 7. Relative fold change in the expression of genes involved in adipogenic differentiation and lipid accumulation of 3T3-L1 preadipocytes exposed to conditioned media from mitotically active (96 h in culture) and differentiated (14 d in culture) satellite cells of beef cattle from different stocker management programs at the conclusion of the stocker and finishing phases

Variable Stocker management program SEM p-value
CON CORN LGWP HGWP
Conditioned media from mitotically active satellite cells
Intermediate harvest
 PPARγ 1.07 1.14 1.08 1.10 0.04 0.5405
 FABP4 0.93 0.88 0.93 0.91 0.03 0.4924
 Oil Red O+ cells (%) 52.43ab 56.04a 51.81ab 47.7b 1.73 0.0082
Final harvest
 PPARγ 1.06b 1.06b 1.09ab 1.16a 0.03 0.0370
 FABP4 0.94a 0.95a 0.92ab 0.86b 0.02 0.0295
 Oil Red O+ cells (%) 57.12 56.35 53.56 54.02 1.50 0.2209
Conditioned media from mitotically active satellite cells
Intermediate harvest
 PPARγ 0.05 0.58 0.27 0.12 0.27 0.4666
 FABP4 0.25 1.13 0.60 0.28 0.29 0.1471
 Oil Red O+ cells (%) 72.09ab 74.01a 75.09a 69.81b 1.50 0.0470
Final harvest
 PPARγ 0.19 0.65 0.04 0.45 0.33 0.4610
 FABP4 0.82 1.24 0.44 0.65 0.24 0.1499
 Oil Red O+ cells (%) 69.18ab 71.68a 65.99bc 63.55c 1.66 0.0027
Least square means within a row lacking a common superscript differ (p ≤ 0.05).
SEM, standard error of the LS mean.
CON, 40% crude protein cottonseed meal-based supplement on dormant native range; CORN, corn and soybean meal-based supplement on dormant native range; LGWP, reduced rate of gain on wheat pasture; HGWP, high rate of gain on wheat pasture