Table 2. Analysis of variance on the treatment conditions affecting the in vitro ruminal parameters of corn flakes using the pressurized steam chamber

Items	df1)	Mean square
		pH	Ammonia	Acetate	Propionate	Butyrate	Total-VFA2)
Trt3)	12	0.11**	96.19**	5,172.05**	7,264.31**	1,201.78**	25,729.68**
Time4)	6	59.28**	3,609.78**	534,471.83**	679,900.33**	273,212.81**	4,249,018.06**
Breed5)	1	0.03*	18.19NS	4,012.75**	24.88NS	6,425.15**	139.79NS
Trt × time	72	0.12**	49.22**	1,240.15**	1,479.41**	573.61**	5,216.24**
Trt × breed	12	0.03**	20.75**	2,196.52**	892.98**	948.34**	2,512.23**
Time × breed	6	0.01NS	44.89**	4,382.86**	1,018.09**	2,381.00**	1,812.62**

Degree of freedom.
Total-VFA, total volatile fatty acids.
T1 (100°C, 720 s); T2 (105°C, 700 s); T3 (105°C, 850 s); T4 (105°C, 950 s); T5 (110°C, 400 s); T6 (112°C, 700 s); T7 (112°C, 850 s); T8 (112°C, 950 s); T9 (113°C, 600 s); T10 (115°C, 500 s); T11 (116°C, 700 s); T12 (116°C, 850 s); and T13 (116°C, 950 s).
Time, in vitro ruminal incubation time.
Breed, Holstein and Hanwoo.
p < 0.05,
p < 0.01,
not significant.