Table 2
Data of DMI, milk production, milk composition, and various serum variables related to carbohydrate metabolism as well as to proinflammatory cytokines and acute phase proteins at the diagnosis week, and prior to the diagnosis of subclinical mastitis (SCM) at -8 and -4 weeks prior to expected day of parturition

	-8 weeks before parturation			4 weeks before parturation			SCM diagnosis weeka			4 weeks after parturition
Item	CON	SCM	P-value	CON	SCM	P-value	CON	SCM	P-value	CON P-value	SCM	P-value
DMI (kg/d)	16.27 ± 1.04	14.94 ± 1.04	0.42	15.93 ± 0.10	12.98 ± 0.75	0.05	20.34 ± 0.56	16.23 ± 0.44	<0.01	21.16 ± 0.68	22.46 ± 1.79	0.23
Milk production (kg/d)							43.01 ± 1.62	28.23 ± 3.95	<0.01	42.04 ± 2.21	35.36 ± 3.96	0.17
Milk composition (g/kg, unless otherwise stated)
Fat							5.08 ± 0.45	3.32 ± 0.41	0.02
Protein							3.00 ± 0.10	2.97 ± 0.09	0.86
Fat : Protein ratio							1.69 ± 0.12	1.11 ± 0.12	<0.01
Lactose							4.54 ± 0.05	4.53 ± 0.06	0.92
SCC (103 cells/mL)							28.33 ± 5.63	1,867 ± 699	0.02
Milk urea N (mg/dL)							15.39 ± 0.76	18.70 ± 1.11	0.03
TS							12.21 ± 0.31	12.19 ± 0.26	0.95
Serum parameters
Lactate (μmol/L)	2,455 ± 349	3,478 ± 153	0.03	2,162 ± 184	3,467 ± 546	0.04	2,228 ± 320	2,215 ± 203	0.98	2,100 ± 129	2,999 ± 76	<0.01
NEFA (mmol/L)	141 ± 32.77	179 ± 42.91	0.51	194 ± 47.17	92.97 ± 10.42	0.09	756 ± 232	303 ± 55	0.11	500.43 ± 151.71	243.89 ± 39.28	0.16
BHBA (μmol/L)	352 ± 37.71	336 ± 42.62	0.78	312 ± 18.50	354 ± 3.32	0.08	827 ± 151	598 ± 176	0.35	896.73 ± 188.3	777.8 ± 91.43	0.58
IL-1 (pg/mL)	316.79 ± 6.04	308 ± 13.76	0.55	321 ± 1.59	306. ± 1.46	<0.01	277. ± 5.42	297 ± 4.50	0.02	270.57 ± 2.87	283.02 ± 7.22	0.14
IL-6 (pg/mL)	19.23 ± 5.67	13.23 ± 3.15	0.42	48.24 ± 17.51	14.75 ± 6.13	0.24	23.17 ± 5.18	23.43 ± 4.89	0.97	15.71 ± 3.27	21.73 ± 6.02	0.38
TNF (ng/mL)	0.34 ± 0.03	0.35 ± 0.02	0.96	0.27 ± 0.05	1.29 ± 0.05	<0.01	0.06 ± 0.03	0.23 ± 0.01	0.01	0.07 ± 0.04	0.28 ± 0.04	0.01
Haptoglobin (mg/mL)	0.19 ± 0.03	0.05 ± 0.01	<0.01	0.15 ± 0.01	0.07 ± 0.01	<0.01	0.12 ± 0.01	0.33 ± 0.02	<0.01	0.16 ± 0.01	0.10 ± 0.02	0.04
SAA (ug/mL)	8,448 ± 3,373	8,799 ± 1,464	0.96	3,461 ± 342	21,052 ± 12,162	0.39	10,401 ± 1722	33,705 ± 9,459	0.05	11,797 ± 1,853	31,419 ± 8,039	0.05