Table 1
The primer sequence used to amplify the exonic sequence variants in the bovine FTO gene

Primer	Primer sequences (5′-3′)	Amplified region	Fragment size (bp)	Location	GenBank accession no.
FTO-1	F-GGCTATAACGGCAGCATGAA	exon 1	844	4 ~ 847	AC_000175.1
	R-CCAGGTGGTGAAGATGGAAA
FTO-2	F-AAGGCATTGTCAGCCTGGATG	exon 2	714	107821 ~ 108536
	R-ATATGGTACAAGGAGCACCAGG
FTO-3	F-TGTCTGTAATGGAGCTTGGACC	exon 3	906	124883 ~ 125788
	R-CAATCATATCTCCAGAGCCTGC
FTO-4	F-CCTTTGCAACTCTCTAGTCCTGTG	exon 4	1064	144220 ~ 145283
	R-CTCTCCAAACTCCCCAAACTTC
FTO-5	F-TTTCCAGGCAAGAGTACTGGAG	exon 5	675	166054 ~ 166728
	R-CTTGACCTTGACCTTGACCTTG
FTO-6	F-AACGTGGGGTTCACTTACTGTG	exon 6	651	175553 ~ 176203
	R-GCAAGAATACTGGAGTGGGTTG
FTO-7	F-CTGTCATGTGCTTTAGGATCTC	exon 7	617	183602 ~ 184218
	R-TGTCTGTGAGTCAACTTCTGTT
FTO-8	F-TAAGGCAGTGGAGCTTTGGA	exon 8	821	235720 ~ 236540
	R-TGTGCACAGAAATGAGGCTG
FTO-9	F-TCACAGTGGATTGCCAAGGT	exon 9	824	420877 ~ 421700
	R-TGTGGCTCATGCAACTGAAG