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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2024.e80

Article

Complete genome sequence of Enterococcus faecium strain AJ_C_05 with potential characteristics to break down carbohydrates, applicable to livestock industry

Yejin Choi1, Hyunok Doo1, Jinok Kwak1, Juyoun Kang1, Eun Sol Kim1, Gi Beom Keum1, Sumin Ryu1, Haram Kim1, Yeongjae Chae1, Sheena Kim1, Hyeun Bum Kim1,*, Ju-Hoon Lee2,**
1Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea.
2Department of Food Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Korea.
**Corresponding Author: Hyeun Bum Kim, Department of Animal Biotechnology, Dankook University, Cheonan 31116, Korea, Republic of. E-mail: hbkim@dankook.ac.kr.
**Corresponding Author: Ju-Hoon Lee, Department of Food Animal Biotechnology, Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Seoul National University, Seoul 08826, Korea, Republic of. E-mail: juhlee@snu.ac.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 25, 2024; Revised: Jul 05, 2024; Accepted: Aug 20, 2024

Published Online: Aug 23, 2024

Abstract

<italic>Enterococcus faecium</italic> (<italic>E.</italic> <italic>faecium</italic>) strain AJ_C_05 was isolated from a Doenjang<italic>, </italic>a traditional Korean fermented food made from soybeans<italic>,</italic> and its complete genome was sequenced using the Oxford MinION platform. The complete genome of <italic>E.</italic> <italic>faecium</italic> strain AJ_C_05 has one circular chromosome and two plasmids. The chromosome of <italic>E.</italic> <italic>faecium</italic> strain AJ_C_05 consists of 2,623,546 base pairs (bp) with a guanine + cytosine (G+C) content of 38.3%, 2,680 coding sequences, 69 tRNAs, and 18 rRNAs. One plasmid is 164,853 bp long and has a G+C content of 35.5%, and the other plasmid is 11,235 bp long with G+C content of 33.1%. <italic>E. faecium</italic> strain AJ_C_05 possesses genes for enzyme determinants, such as alpha-galactosidase (EC 3.2.1.22), beta-glucosidase (EC 3.2.1.21), cellulases (EC 3.2.1.4), chitinase (EC 3.2.1.122), proteases (EC 3.4.21.112), and xylanase (EC 3.2.1.8). These characteristics of <italic>E. faecium</italic> strain AJ_C_05 appears to indicate its potential to be used as probiotic feed additives. This study offers valuable insight into the functionality of <italic>E. faecium</italic> isolated from soybean paste, which has the potential to promote digestion when used as a feed additive.

Keywords: Whole genome sequencing; Enterococcus faecium; Feed additive