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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2024.e75

Article

Effects of MTAP and PMEL gene Polymorphisms on Plumage Color Variation in Chickens

Jean Pierre Munyaneza1, Eunjin Cho2, Minjun Kim1, Daehyeok Jin3, Jihye Cha4,*, Jun Heon Lee1,2,**
1Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea.
2Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea.
3Animal Genetic Resources Research Center, National Institute of Animal Science, Rural Development Administration, Hamyang 50000, Korea.
4Animal Genome & Bioinformatics, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea.
*Corresponding Author: Jihye Cha, Animal Genome & Bioinformatics, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea, Republic of. Phone: +82-63-238-7305. E-mail: wischa91@korea.kr.
**Corresponding Author: Jun Heon Lee, Division of Animal and Dairy Science, Chungnam National University, Daejeon 34134, Korea, Republic of. Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Korea, Republic of. Phone: + 82-42-821-7031. E-mail: junheon@cnu.ac.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 19, 2024; Revised: Jul 17, 2024; Accepted: Jul 26, 2024

Published Online: Jul 30, 2024

Abstract

Plumage color is an important economic trait in chickens and is mainly affected by genetic factors than environmental factors. This study aimed to detect the single-nucleotide polymorphisms (SNPs) in <italic>CDKN2A</italic>, <italic>MTAP</italic>, and <italic>PMEL</italic> genes and explore their influence on plumage color variation in chickens. We used 428 chicken blood samples, consisting of all-black: 62, all-white: 246, and black and white barred: 120 chickens of F2 population produced from crossing the F1 progenies. The F1 population was produced by crossing Yeonsan Ogye (YO) and White Leghorn (WL). The SNPs in the <italic>CDKN2A</italic>, <italic>MTAP</italic>, and <italic>PMEL</italic> genes were initially detected by sequencing. PACE Genotyping technology was used for genotyping and results were observed for a synonymous SNP, rs316391660C/T of the <italic>MTAP</italic> gene, missense SNPs, rs312616138A/G and rs14684281T/C of the <italic>PMEL</italic> gene. The association test between the genotypes in <italic>MTAP</italic> (SNP: rs316391660C/T) and <italic>PMEL</italic> (SNP: rs14684281T/C) genes was performed by Chi-square test while Fisher’s exact test to evaluate association the genotypes of PMEL gene (SNP: rs312616138A/G) with plumage color variations. The missense SNP, rs1058656732C/T in <italic>CDKN2A</italic> gene was monomorphic and could not be used for the association test. There was a significant (p&lt;0.05) association between genotypes of MTAP and PMEL genes with the three plumage color variations: all-black, all-white, and black and white barred. Our results confirm the genotype effects of the PMEL gene on the dominant white plumage color, and suggest that the synonymous SNP (rs316391660C/T) of the MTAP gene could be used as a genetic marker for the breeding of chickens with black-and-white barred plumage.

Keywords: MTAP gene; Plumage color; PMEL gene; F2 population