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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2024.e65

Article

The Role of Extracellular Vesicles in Murine Asthma Model: Insights into IgE-Independent Mast Cell Activation within Animal Science

Hyun-Woo Kim1, Ji-Su Kim2, Yejun Seo3, Young Rak Kim3, Changdong Kim3, Jin-Hyeon Hwang1, Seong-Kyoung Kim1, Jun-Gu Kang1, Jeong-Yoon Lee1, Kyung-Tae Lee1, Jae-Seok Jeong1, Moon-Chang Baek4, Young-Eun Cho2,*, Do-Kyun Kim1,**
1Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Korea.
2Department of Food and Nutrition, Andong National University, Andong 36729, Korea.
3Andong high school, Andong 36753, Korea.
4Department of Molecular Medicine, CMRI, School of Medicine, Kyungpook National University, Daegu 41944, Korea.
*Corresponding Author: Young-Eun Cho, Department of Food and Nutrition, Andong National University, Andong 36729, Korea, Republic of. E-mail: yecho@anu.ac.kr .
**Corresponding Author: Do-Kyun Kim, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Korea, Republic of. E-mail: dkkim714@jbnu.ac.kr .

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: May 09, 2024; Revised: Jun 13, 2024; Accepted: Jun 21, 2024

Published Online: Jun 21, 2024

Abstract

Asthma, a prevalent respiratory condition in animal science, involves bronchial inflammation, leading to symptoms like coughing and difficulty breathing. Mast cells and macrophages, pivotal immune cells, contribute to the inflammatory response triggered by various factors. Extracellular vesicles (EVs), including exosomes, play crucial roles in intercellular communication and have been implicated in murine asthma. We hypothesize that murine asthma-derived EVs modulate immune cell responses in animals. Our study investigates the role of these EVs in immune cell activation, shedding light on asthma pathogenesis. Using a murine asthma model induced by ovalbumin (OVA), we assessed serum EVs via Nanoparticle Tracking Analysis (NTA). Serum EVs from normal and asthmatic mice were are subjected to ELISA (Enzyme-linked immunosorbent assay) and proteomic analysis for component identification. Elevated EV concentration and inflammatory cytokines, including such as Interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α, were are found in asthma-derived EVs. Additionally, variations in immunoglobulin light chain types were are identified. Surprisingly, murine asthma EVs failed to activate T lymphocytes, B lymphocytes, eosinophils, and macrophages but stimulated mouse bone marrow-derived mast cells (mBMMCs) via enhanced degranulation and MAP kinase phosphorylation. These results suggest murine asthma-derived EVs as potential therapeutic targets for managing asthmatic symptoms in animal science. Further research into their mechanisms and clinical applications is warranted.

Keywords: Extracellular vesicles; Murine Asthma Model; Mouse Bone Marrow-derived Mast Cells (mBMMCs); MAP kinase phosphorylation; Animal Science