Dangguibuxue Decoction Protects Against Lipopolysaccharides-Induced Mastitis in bovine mammary epithelial cells in Vitro

Jiamian Wang¹^,², Yujin Gao¹^,², Chen Cheng¹^,², Yina Li¹^,², Xijun Zhang¹^,², Dan Yao¹^,², Yong Zhang¹^,²^,^*

¹College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.

² Gansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou 730070, China.

^*Corresponding Author: Yong Zhang, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. Gansu Key Laboratory of Animal Reproductive Physiology and Reproductive Regulation, Lanzhou 730070, China. E-mail: zhychy@163.com.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Mar 26, 2024; Revised: May 27, 2024; Accepted: Jun 16, 2024

Published Online: Jun 17, 2024

Abstract

Bovine mastitis (BM) is a prevalent and recurring disease in dairy farming. Danggui Buxue Decoction (DBD) possesses significant anti-inflammatory, antioxidative stress, and immune system regulatory effects. However, its application in the clinical prevention and treatment of bovine mastitis has not been explored. This study aimed to assess the effectiveness and mechanism of DBD on bovine mastitis through an in vitro model using lipopolysaccharide (LPS)-stimulated bovine mammary epithelial cells (BMECs). LPS stimulation led to increased expression of inflammatory markers (TNF-α, IL-1β, IL-6, and IL-8), damage indicators (BNBD5, GM-CSF, CCL2, and GADD-45α), and oxidative stress markers (COX-2, PPARγ, and iNOS) in the BMECs. Conversely, treatment with DBD counteracted these effects. Additionally, a lactate dehydrogenase (LDH) release assay indicated that DBD diminished the cellular damage caused by LPS. The Total antioxidant capacity (T-AOC), Glutathione (GSH), Superoxide dismutase (SOD), Malondialdehyde (MDA), Nitric Oxide (NO) content assay, and Reactive Oxygen Species (ROS) staining demonstrated that DBD mitigated cellular oxidative stress triggered by LPS. Moreover, DBD suppressed the mRNA and protein expression of TLR4 and NF-κB. These results imply that DBD exerts anti-inflammatory, wound healing, and antioxidative stress effects on BMECs following LPS exposure, suggesting its potential as an effective treatment for bovine mastitis.

Keywords: Bovine mastitis (BM); Danggui Buxue Decoction (DBD); bovine mammary epithelial cells (BMECs); toll-like receptor 4 (TLR4); inflammation