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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2024.e62

Article

Complete genome sequence of bacteriocin-producing Lactococcus lactis subsp. lactis DOME 6301 with potential oral-pathogen control applications

Arxel Elnar1, Yujin Jang1, Byeong-Gwan Eum1, Yookyung Hur2, Chul Sung Huh2,3, Geun-Bae Kim1,*
1Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
2Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea.
3Bio Dome, 145, Jijeong-myeon, Wonju 26355, Korea.
*Corresponding Author: Geun-Bae Kim, Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea, Republic of. Phone: +82-31-670-3027. E-mail: kimgeun@cau.ac.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Apr 11, 2024; Revised: Jun 10, 2024; Accepted: Jun 12, 2024

Published Online: Jun 13, 2024

Abstract

<italic>Lactococcus lactis </italic>subsp. <italic>lactis </italic>DOME 6301, isolated from bovine milk, produces an antimicrobial compound that inhibits oral pathogens including <italic>Streptococcus mutans</italic>, <italic>Prevotella intermedia</italic>, and <italic>Fusobacterium nucleatum</italic>. The entire genome of <italic>L. lactis</italic> DOME 6301 was sequenced and assembled <italic>de novo </italic>using the PacBio RS II platform. The genome was 2,532,858 bp in length, assembled into three contigs, and had a guanine and cytosine (G + C) ratio of 35%. The annotation results revealed 2,469 protein-coding sequences, 22 rRNA genes, and 78 tRNA genes. Genes involved in the utilization of complex carbohydrates (i.e., cellulose, xylose, pullulan, amylose, maltodextrin, and arabinofuranose) and synthesis of the bacteriocin, nisin Z, were detected. In addition, genes encoding antimicrobial resistance and virulence factors (i.e., hemolysin and enterotoxin) were detected. Whole-genome analysis of strain DOME 6301 contributed to our understanding of the evolution of the taxa and provided the basis for the correct selection of probiotic candidates for downstream applications.

Keywords: Lactococcus lactis subsp. lactis; genome announcement; nisin Z bacteriocin; oral pathogens; functional dairy products