Antimicrobial Activity of Pediococcus pentosaceus Strains against Diarrheal Pathogens Isolated from Pigs and Effect on Paracellular Permeability of HT-29 cells

Eunbee Cho¹, Yoonjeong Yoo¹, Yohan Yoon¹^,^*

¹Sookmyung women's university, Seoul 03948, Korea.

^*Corresponding Author: Yohan Yoon, Sookmyung women's university, Seoul 03948, Korea, Republic of. E-mail: yyoon@sookmyung.ac.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jan 19, 2024; Revised: Mar 27, 2024; Accepted: Apr 22, 2024

Published Online: Apr 22, 2024

Abstract

This study aimed to investigate lactic acid bacteria with antimicrobial activities against infectious diarrheal pathogens in pigs and their genetic characteristics. Acid-resistant lactic acid bacteria were examined for bile resistance, pancreatic enzyme resistance, gelatinase and urease activities, and antibiotic resistance. Subsequently, selected isolates were examined for antimicrobial activities against <italic>Campylobacter coli</italic>,<italic> Clostridium perfringens</italic>, <italic>Escherichia coli</italic>, and <italic>Salmonella</italic> Typhimurium, and their effects on paracellular permeability and the expression of tight junction protein-encoding genes in HT-29 cells were assessed. Whole genome sequencing was performed to identify the genes related to safety and antibacterial activity. Of the 51 isolates examined, 12 were resistant to bile and pancreatin and did not produce gelatinase and urease. Of these 12, isolates 19, 20, 30, 36, and 67 showed tetracycline resistance and isolates 15, 19, and 38W showed antimicrobial activity against infectious diarrheal bacteria. Treatment with isolate 38W signiﬁcantly reduced the paracellular permeability induced by <italic>E. coli </italic>in HT-29 cells and alleviated the expression of tight junction protein-encoding genes (<italic>claudin-1</italic>, <italic>occludin,</italic> and <italic>ZO-1</italic>) induced by <italic>E. coli </italic>inoculation. Isolates 15, 19, and 38W were named as <italic>Pediococcus pentosaceus</italic> SMFM2016-NK1, SMFM2016-YK1, and SMFM2016-WK1, respectively. Bacteriocin-related genes were <italic>YheH, ytrF, BceA, BceB</italic>, and <italic>MccF</italic> in SMFM2016-NK1; <italic>YheH, ytrF, BceA, BceB, entK, lcnA, MccF</italic>, and <italic>skgD</italic> in SMFM2016-YK1; and <italic>YheH, ytrF, BceA, BceB</italic>, and <italic>MccF</italic> in SMFM2016-WK1. SMFM2016-YK1 harbored the <italic>tetM</italic> gene. These results indicate that <italic>P. pentosaceus</italic> SMFM2016-WK1 might control diarrheal pathogens isolated from pigs. However, a further study is necessary because the results were obtained only from <italic>in vitro</italic> experiment.

Keywords: Antimicrobial agent; Feed additive; Probiotics; Gut health; Lactic acid bacteria