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Journal of Animal Science and Technology
Korean Society of Animal Science and Technology

J Anim Sci Technol

pISSN: 2672-0191, eISSN: 2055-0391

DOI: https://doi.org/10.5187/jast.2024.e26

Article

Paralichthys Olivaceus Egg Extract Improves Porcine Oocyte Quality by Decreasing Oxidative Stress

Seung-Eun Lee1,2, Hyo-Jin Park1,2, Dong-Hun Han1,2, Eun-Seo Lim1,2, Han-Bi Lee1,2, Jae-Wook Yoon1,2, Chan-Oh Park1,2, So-Hee Kim1,2, Seung-Hwan Oh1,2, Do-Geon Lee1,2, Da-Bin Pyeon1,2, Eun-Young Kim1,2,3, Se-Pill Park1,3,4,*
1Stem Cell Research Center, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju-do 63243, Korea.
2Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju-do 63243, Korea.
3Mirae Cell Bio, 1502 isbiz-tower 147, Seongsui-ro, Seongdong-gu, Seoul 04795, Korea.
4Department of Bio Medical Informatic, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju-do 63243, Korea.
*Corresponding Author: Se-Pill Park, Stem Cell Research Center, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju-do 63243, Korea, Republic of. Mirae Cell Bio, 1502 isbiz-tower 147, Seongsui-ro, Seongdong-gu, Seoul 04795, Korea, Republic of. Department of Bio Medical Informatic, College of Applied Life Sciences, Jeju National University, 102 Jejudaehak-ro, Jeju-si, Jeju-do 63243, Korea, Republic of. E-mail: sppark@jejunu.ac.kr.

© Copyright 2024 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Nov 24, 2023; Revised: Jan 31, 2024; Accepted: Feb 19, 2024

Published Online: Feb 20, 2024

Abstract

This study aimed to assess the influence of <italic>Paralichthys olivaceus</italic> egg extract (POEE) treatment on the maturation and development of porcine oocytes subjected to oxidative stress during<italic> in vitro</italic> maturation (IVM). POEE, notably rich in vitamin B9 (folic acid, FA), was assessed alongside FA for antioxidant activity across various concentrations. In the 650 ppm POEE (650 POEE) group, there was a significant rise in GSH levels and an improved developmental rate in porcine oocytes experiencing oxidative stress during IVM. Treatment with 0.3 FA exhibited substantial reduction in ROS activity. Both 650 POEE and 0.3 FA groups demonstrated inhibited abnormal spindle organization and chromosomal misalignment, with increased blastocyst formation and decreased apoptotic cells. Treatment with 650 POEE elevated mRNA expression of development-related genes (<italic>SOX2, NANOG,</italic> and <italic>POU5F1</italic>). In conclusion, POEE effectively mitigates oxidative stress, enhances embryonic quality, and improves developmental potential in porcine oocytes on <italic>in vitro</italic> maturation (IVM).

Keywords: Porcine oocyte; Paralichthys olivaceus egg extract; Folic acid; In vitro maturation; Antioxidant activity