Dietary inclusion of glucose oxidase supplementation to corn-wheat-based diet enhance growth performance, nutrient digestibility, blood profile of lactating sows

INTRODUCTION

Modern livestock industries aim to increase the production performance through the selection of sows with large litter sizes. However, during the gestation and lactation period sows have to tackle various stress factors such as body weight loss and changes in their housing [1,2]. Besides, these anxieties may reduce their immune function and increase the risk of pathogenic disease susceptibility in their intestinal tract, which leads to huge economic loss in swine production [3]. Furthermore, the biological and immunological health status of highly producing sows, not only affects their progeny performance but also affects their reproductive performance. To defeat this situation antibiotic growth promoters (AGP) have been used in swine feed for many years to improve their health and feed intake but the use of AGP in livestock feed causes various health issues to animals as well to consumers due to its antimicrobial resistance [4]. Owing to the demand for high quality livestock products, it is important to explore effective and natural feed additives that can stimulate productivity as well improve the health status of swine. Consequently, many researchers were a quest to find a suitable nutritional strategy that can enhance the health status of sows as well as livestock production. In such research, glucose oxidase (GOx) was found to be an excellent alternative

GOx is a small stable enzyme that could oxidizes gluconolactone, oxygen into hydrogen peroxide [5]. They are predominantly produced from Aspergillus niger and Penicilliumglaucum through the fermentation method [6] and found on the surface of fungi. It plays a vital role in a commercial application such as increasing the texture and color of food materials, palate, and shelf life of food products [7] and helps to prevent bacterial infection. During the fermentation processes, GOx could be used as a biosensor to determine D-glucose content in body fluids, foodstuffs, and beverages [8,9]. Due to the high production cost and low fermentation capacity, the use of GOx in livestock feed is not vigorously implemented. Previously, Biagi et al. [10] and Tang et al. [11] pointed out that GOx supplement had a beneficial effect on the growth performance of piglets. Also, Wu et al. [12] demonstrated that the GOx supplement has significantly increased the growth performance of average daily gain (ADG) and feed and gain ratio (G:F) of broilers. Earlier studies have shown that GOx supplement could reduce oxidative stress and intestinal mycotoxin poisoning thereby, improving the immunity to ameliorate the growth performance and productivity of animals [13–15]. Wheat contains variable amounts of non-starch polysaccharides (NSP), main arabinoxylans which can interfere with nutrient digestibility, feed efficiency, and growth performance of poultry and piglets [16,17], such wheat has not been commonly used in sows diet. Though previous literature showed a positive effect on the application of GOx in the livestock industry, still now there was no information presented on the supplementation of GOx with corn-wheat-based diet. Therefore, we initiate this study to evaluate the effect of GOx with corn-wheat diet on the growth performance, nutrient digestibility, and blood profile of lactating sows. We hypothesized that the GOx supplement with corn-wheat-based diet could improve the nutrient digestibility and blood profile of lactating sows and the growth performance of piglets.

MATERIALS AND METHODS

The protocol of this experiment was reviewed and approved by the Animal Care and Use Committee of Dankook University (DK-2-1942) Cheonan, Korea. The source of GOx supplement was obtained from the commercial company (Jinan Bestzyme-Bio Engineering, Jinan, China) and expressed by Aspergillus niger. One unit (U) of GOx activity is defined as the amount of enzyme which oxidates 1 μmol β-D-glucose per minute to D-gluconic acid and H₂O₂ at 37°C and pH 5.5.

A total of 20 multiparous sows (Landrace × Yorkshire) with an average parity of 2.8 (SD = 0.89) (In brief, Parity 1 consist of 1 sow, whereas parity 2 and 3 consists 7 sows, and parity 4 consist-5 sows) were randomly allocated into one of four treatments with five replicates per treatment. The dietary treatments were as follows: CON (basal diet), GO1 (basal diet + 200 U GOx/kg), GO2 (basal diet + 300 U GOx/kg), GO3 (basal diet + 400 U Gox/kg). During gestation period, the sows were caged in separate stalls, which had partially slatted and specific strips floor consisting of a 0.80 × 1.05 m. Experimental diets were fed from day 100 to day 135 of the feeding trial, sows were weighed and transferred to farrowing room at 107th day of gestation, and fed 2.5 kg per day feed to allow for adjustment to the lactation diet before parturition. However, sows were not fed on the day of farrowing. The nutrient diets were formulated to meet or exceed the nutrition criteria of NRC, 2012 (Table 1 [gestation] & Table 2 [lactation]). The farrowing crate contained an air-conditioned for newborn pigs at the same time temperature of the farrowing house was maintained at least 20°C with additional ventilation generated by heat lamps. Within 24 h of birth, all piglets were treated with 1 mL of iron injection, ear notching, needle teeth clipping and tail docking. within 5 days of postpartum male piglets were castrated. During lactation the feed intake of sow had raised up to 7 kg, until day 21 the piglets were continued to weaned in the farrowing room. Water was available ad libitum to both the sows and piglets throughout the experimental. Piglets were not offered creep feed. Sow milk was the only feed available to the piglets during lactation.

At the beginning of the experiment, before farrowing, after farrowing, and at the weaning period individual sows body weight and backfat thickness were measured using real-time ultrasound instruments (Pig lot 105, SFK Technology, Copenhagen, Denmark). After farrowing litter size was recorded according to numbers of alive piglets or dead litter to calculate the survival ratio. Feed consumption and residual were measured after feeding to calculate the daily feed intake of sows’ Body condition score was recorded within a few hours, a day after farrowing, after farrowing to weaning, and weaning to 21-day of lactation. Each piglet body weight was measured at initial birth and 21 days of lactating (weaning). During gestation and lactation, the consumption of feed was recorded on each pen to calculate the ADG, average daily feed intake (ADFI). In order to determine the survival rate, the piglets were recorded to report on farrowing day to weaning day. Each sow weaning estrus interval was noted after weaning. After weaning, sows were transferred to pens, which is very near to mature boar and also, they have direct exposer two times a day (08:00 and 16:00 h) for estrus detection. The presence of a boar, a sow was assumed to be in estrus when displaying a standing reaction caused by a back-pressure test.

To calculate total tract digestibility of dry matter (DM), nitrogen (N), and energy (E), 0.20% chromium oxide was added to the diet as an indigestible marker for 7 days prior to fecal collection at end of the lactation period. Sows rectum was gently massaged by the trainer and fresh fecal samples were collected, pooled (pen basis) and stored at −20°C until analyzed. All feed and fecal samples were freeze-dried and finely ground to pass through a 1 mm screen. DM and N digestibility were determined using methods established by the Association of Official Analytical Chemists [18]. UV absorption spectrophotometry (UV-1201, Shimadzu, Kyoto, Japan) was used to determine the chromium absorption in the diets and feces. Parr 6100 oxygen bomb calorimeter (Parr Instrument, Moline, IL, USA) was used to analyze energy by measuring the heat of combustion in the samples. N was analyzed using Kjeltec 8600 (Foss Tecator AB, Hoeganaes, Sweden). The calculation of total tract digestibility formula was used according to Sales and Janssens [19]. The blood samples were collected via puncturing the vena cava of sows before feeding at farrowing, after farrowing, and at weaning. For serum analysis, approximately 3 mL blood samples were centrifuged at 4,000 (rpm)×g for 15 min at 4°C to obtain serum samples and then stored at −20°C until analysis. The serum antioxidant activities of glutathione peroxidase (GPX), glutathione (GSH), and glutathione disulfide (GSSG) were detected using the ELISA kit (R & D Systems, Minneapolis, MN, USA). Superoxide dismutase (SOD) in serum was measured using a commercial kit from Cayman Chemical (Ann Arbor, MI, USA). The blood glucose concentrations were analyzed by an automatic biochemistry blood analyzer (HITACHI 747, Hitachi, Tokyo, Japan).

RESULTS AND DISCUSSION

Dietary inclusion of GOx supplement in the diet of lactating sows failed to affect the reproductive performance as well as body weight, backfat thickness, and body condition score before farrowing, after farrowing, and weaning (21 days) (p > 0.05) (Table 3). However, from farrowing to weaning, lactating sow’s bodyweight loss was linearly decreased in the GOx treatment groups (p = 0.020). Moreover, there were no effects on sow backfat thickness loss, ADFI, and estrus interval among the treatments (p > 0.05). Previously, Wu et al. [12] reported that dietary inclusion of GOx supplementation had significantly enhanced growth performance and gut health of chicken. Moreover, Baudon and Hancock [20] demonstrated that low feed intake results sows with excessive body weight loss. Furthermore, Bergsmaet al. [21] pointed out that decreased feed intake during the lactation period may leads to excessive body weight loss of sows that can decrease milk production and subsequent litter development. Whilst, Eissen et al. [22] demonstrated that enhanced feed intake during lactation can help the sow to reduce back fat thickness loss and body weight loss. However, in this study, there were no significant difference (p <0.05) observed on the ADFI and body weight loss (BWL) in sows before farrowing and after farrowing, and at weaning (21 days). To date, no literature has been presented on the application of GOx in sow diet. However, Tang et al. [23] noted that the dietary inclusion of GOx supplementation has increased growth hormone content, and modified the fecal microbiota, thereby improving he growth efficiency in weaned piglets fed a corn-based diet. Previously Gatrell et al. [24] pointed out the supplementation of corn and wheat as a dominant energy sources in poultry diets due to their high energy content. However, the presence of NSP in wheat and corn may negatively affect nutrient utilization and performance of monogastric animal [25,26]. Apart from this, the NSP-degrading enzymes in diets has been shown to improve apparent ileal digestibility of NSP constituents, other than galactose [27], and this, in turn, results in improved digestibility of organic matter, amino acids and energy [28,29]. However, with an opposing result, Han et al. [30] stated that pigs fed wheat-based diet has improved growth performance and meat quality of pigs. Similarly, Seerly et al. [31] and Van Lunen and Schulze [32] reported that pigs fed wheat-based diet had a significantly higher ADFI than those fed a corn-based diet. Besides, some researchers reported that there was no significant difference between corn and wheat diets [33]. We assume that the discrepancies results might be due the difference on animals age or due to the concentration of wheat-based diet.

In an earlier study Park et al. [34] stated that a high-energy diet has reduced the body weight (BW) of sows and enhance the growth performance of piglets until weaning period. The present study indicates that GOx supplementation had no linear effects on the initial number and final number of suckling piglets, and their survival rate (p > 0.05). However, sows fed GOx supplementation has a beneficial effect on the growth performance of their piglets by linearly increasing their BW during the weaning period (p = 0.049). In addition, linearly enhanced ADG (p = 0.04) was observed on piglets during the overall experiment period (Table 4) was agreed with Biagi et al. [10] who reported that GOx supplementation has enhanced the growth performance of piglet’s especially after weaning. Likewise, Tang et al. [15] reported that adding 100 U/1 kg GOx supplement to soybean meal-based diet had enhanced the BWG and feed conversion ratio of piglets. Also, Mu et al. [35] stated that the dietary inclusion of 0.04% GOx (250 U/g) had enhanced the SOD concentrations, serum glutathione, and growth performance of weaning pigs. According to Cabrera et al. [36] weaning weight of piglet has a direct effect on their post weaner performance. After weaning, pigs with high weaning weight can grow faster than those with lighter weaning weight [37]. The growth performance can represent a beneficial impact on the digestibility of nutrients. However, the present study did not assess the nutrient digestibility of suckling piglets.

At the end of this experiment, the apparent total digestibility of DM (p = 0.045), and N (p = 0.084) in lactating sows showed a linear, and trend to increase with a graded level of GOx. However, there were no effects observed on energy (p > 0.05) digestibility (Table 5). Previously, many researchers had reported that the pigs fed cellulose or enzyme mixture supplementation has improved the apparent nutrient digestibility of DM, energy, and crude protein, thereby enhancing the growth performance of pigs [38–40]. In addition, Wu et al. [12] demonstrated that total tract digestibility was regulated by the intestinal microbiota. As reported by Liu et al. [41], effective nutrient absorption and better performance are usually associated with enhanced villus height and decreased crypt depth. However, the present study has failed to measure intestinal morphology. Therefore, we assume that the enhanced nutrient digestibility (with GOx) may be associated with intestinal microflora. There were limited research results available on the enhanced performance of lactating sows’ nutrient digestion with GOx supplement, so we could not able to make more comparison with other studies.

Dietary supplementation of GOx linearly improved SOD (p = 0.050) after farrowing. Similarly, weaning period lactating sows had a linear effect on observed SOD, and glucose (p = 0.049, and 0.042, respectively), without effects on GPx, GSSG, and GSH in GOx-supplementation diets (p > 0.05) (Table 6). Yoon et al. [42] reported that dietary inclusion of 0.05% β-mannanase enhanced the blood profile glucose level in finishing pigs. In contrast, Tan et al. [43] stated that dietary inclusion of corn starch supplementation significantly reduced the serum glucose in the sow’s gestation and lactation period and improved ADFI. In, 2019, Yang et al [44] demonstrated that glucose receptors are present in the brains center, and a reduce in the plasma glucose concentration of corn-starch fed sows possibly means the decline of neuronal signaling for the metabolism of glucose, thereby promoting sow feed intake. During normal respiration in mitochondria, the biological system could produce superoxide, which might have strong damage to the organism and cells. Fan et al. [45] demonstrated that the anti-oxidant ability of sows at farrowing was mainly modulated by SOD, which consists of sows’ defense system with other anti-oxidant enzymes in order to manage the oxidative damage caused by excessive activists. Blood profiles of SOD, GPX, GSH, and GSSG is considered the most representative markers of oxidation status invivo [46]. Waisundara [47] reported that an increase in antioxidant enzyme level could reduce oxidative stress. An early study, Michiels et al. [48] demonstrated that the oxidative state of the weaned piglet has been found to influence factors such as birth weight. GSH acts as a major endogenous antioxidant with respect to the gut, GPX transforms GSH to its GSSG oxidized type. Oxidative stress will increase the GSSG level along with a corresponding decrease in SOD, GPX, and GSH concentrations [49,50]. Therefore, we believe that dietary inclusion of GOx has been shown to improve blood profile glucose and SOD of lactating sows by enhancing the immune system. However, there was no information presented on the effects of GOx supplementation on the blood profile of lactating sows’ diets, so adequate justifications could not be made. Thus, more trials are needed to know the exact cause for the lack of results in this study.

CONCLUSION

Our findings demonstrated that the inclusion of GOx supplement to corn-wheat-based diet had a beneficial effect on the reproductive performance of lactating sows, as well as it enhanced the growth performance of body weight and ADG of suckling piglets. In addition, gradually increased levels of GOx supplementation in the diet of lactating sows had linearly improved DM, N digestibility, and blood profile. Besides, current results will provide a novel insight on the applications of the GOx diet as an excellent alternative solution to promote the growth efficiency of lactating sows in the future.