Replacement value of cottonseed meal for soybean meal in broiler chicken diets with or without microbial enzymes

Four wheat/sorghum/soybean-based diets were formulated using the least-cost method to meet the nutrient specifications for Ross 308 broiler chicks Aviagen [13]. The diets contained different levels of CSM as follows: control (0%) in all growing phases (starter, grower, and finisher), low (4%, 8%, and 12%), medium (5%, 10%, and 15%) and high (6%, 12%, and 18%) respectively. Each diet was fed as such or supplemented with composite microbial enzymes (Axtra XB) at 100 mg/kg of diet. Axtra XB (Danisco Animal Nutrition, UK) is a blend of two microbial enzymes, xylanase and β-glucanase, providing 250 units of β-glucanase and 2,500 units of xylanase per kg of the formulated feeds. In addition, an indigestible marker, titanium dioxide (TiO₂), was incorporated in the grower diets at a rate of 5 g/kg of diet, in order to assess nutrient digestibility. The starter chicks (from one to 10 days) were fed with crumbled feed, while the grower (from 11 to 24 days) and finisher (from 25 to 35 days) chicks were fed whole pellet diets. Diets were pelleted at 65°C. Tables 1 and 2 show the ingredient composition of the diets used in the trial and the calculated nutrient composition of these diets respectively, while Table 3 shows the analyzed nutrient composition of the tested CSM, which were locally produced and purchased (Riverina, Milton, QLD, Australia).

The trial was a 4 × 2 factorial arrangement in which birds were randomly allocated into eight treatments. Each treatment was replicated six times, with 10 chickens per replicate, which made a total of 480 birds. One-day old male Ross 308 chicks were purchased from a commercial hatchery, (Baiada, Tamworth NSW, Australia), with average initial weight of 41.1 ± 0.8 g/chicks. The cages were placed in a climate controlled room. Light was provided for 24 h on the first day and then reduced to 18 h per day from the second day until the end of the experiment. The room temperature was also set as follows: 33°C on day one, then gradually decreased to 24°C from day 18 to the end of the feeding trial. Each cage was provided with a feeding trough and automated water system with two drinking nipples. The birds had ad libitum access to drinking water and feed. Bird observations were done on a regular basis to monitor health status, feed and water supplies, and environmental conditions. The experiment was ended at 35 days of age.

Feeds and birds were weighed at a cage basis on 0, 10, 24, and 35 days of age to determine the FI, WG, and feed conversion ratio (FCR). In addition, when there was a death, the dead bird, the remaining birds and feeds were weighed to correct FI and FCR. The visceral organs (small intestine, liver, gizzard and proventriculus, bursa and spleen) were collected from the birds killed on day 10 and 24 weighed and recorded. The relative weight of these organs was calculated as g/100g of body weight. To determine apparent nutrient digestibility in the ileum, diets and digesta samples were collected. Diet samples were collected in plastic bags directly from the feed mill after pelleting and cooling, ground and kept in plastic containers for nutrient analysis. On the 24th day of the trial, three birds were randomly selected from each replicate, weighed and euthanized by cervical dislocation to collect digesta samples. The entire gastrointestinal tract (GIT) was exposed and the ileum, which is the small intestinal portion extending from the vitelline (Merkel’s) diverticulum to a point about 40 mm above the ileo-caecal junction, was cut and its contents were then flushed into a plastic container, frozen and freeze dried. Lyophilized ileal digesta were then ground using a coffee grinder and kept in air-tight containers at 4°C for further analysis.

To determine the carcass yield, on day 35, two chickens were chosen from each cage, weighed, electrically stunned and euthanized using the cervical dislocation technique. The bird was scalded for 30 sec in hot water (approximately 63°C), and their feathers were plucked out in a batch defeatherer after which the carcass was eviscerated. The entire breast meat (pectoralis minor and pectoralis major), wings, thighs, and drumsticks (bones included) were separated from the carcass and their weight was recorded. The weight of these carcass parts was expressed as g/kg of bird live weight. The remaining carcass parts and feeds were disposed of according to the local regulations.

The concentration of TiO₂ in the diets and ileal digesta was measured according to the colorimetric procedure of Short et al. [14], using about 0.1 g of digesta and 0.2 g of the diet samples. Ground diet samples were oven-dried at 105°C for 24 h to determine DM content of experimental diets using a forced air convection oven (Qualtex Universal Series 2000, Watson Victor Ltd, Perth, Australia). The DM content of the ileal digesta samples was obtained directly after freeze-drying (Martin Christ freeze dryer, Germany) at −50°C for at least 72 h. The nitrogen content of ileal digesta and diet samples was measured using the Dumas combustion technique, following the method described by Sweeney [15], through direct electronic reading on a LECO® FP-2000 automatic nitrogen analyzer (Leco Corporation, St. Joseph, MI, USA). The obtained nitrogen results were then converted to CP content, using a factor of 6.25. Around 0.5 g of finely ground (0.5 mm) diet and ileal digesta samples were weighed in metallic crucibles to determine gross energy (GE), using an IKA®-WERKE bomb calorimeter (C7000, GMBH & Co., Staufen, Germany), and the GE value of samples measured as MJ/kg, was read from the calorimeter digital system. The method developed by McCleary et al. [16] was followed to assess the total starch content of diets and ileal digesta. The Megazyme total starch assay kitAA/AMG (Megazyme International Ireland Limited, Bray, Co. Wicklow, Ireland) was used for total starch analysis. Absorbance was read at 510 nm on a spectrophotometer (Varian Cary 50 Bio UV – Visible Spectrophotometer Mulgrave, Victoria, Australia) against milli-Q water.

The index method proposed by Moughan and Marlies Leenaars [17] and reviewed by Kong and Adeola [18] with titanium dioxide as an index compound was used to determine nutrient digestibility. The following equation was used to calculate apparent ileal digestibility of nutrients:

where, AIDN is the coefficient of apparent ileal digestibility of nutrients, N_Digesta is the nutrient concentration in digesta (g/kg), TiO_{2 Digesta} is the titanium concentration in digesta (g/kg), N_Diet is nutrient concentration in diets (g/kg) and TiO_{2 Diet} is the titanium concentration in feeds (g/kg).

Broiler chicken’s growth, visceral organs development, meat yield and nutrient digestibility data were statistically analyzed according to 4 × 2 factorial arrangement. The obtained data were subjected to analysis of variance (ANOVA) in the procedures of general linear model of Minitab statistical software, version 17 [19]. Fisher’s least significant difference (LSD) method for multiple comparisons was used to separate significant differences between mean values at p ≤ 0.05 level of probability.

The experimental design and methodology were approved by the Animal Ethics Committee of the University of New England, Australia under approval number AEC15-084. All husbandry and management procedures used were in accordance with The Code of Practice for the Use of Animals for Scientific Purposes was issued by the Australian Bureau of Animal Health.

The FI, WG, and FCR in response to the four CSM levels in diets supplemented with or without Axtra XB enzyme are summarized in Table 4. In starter broilers (1–10 days), there was an interaction between the enzyme and CSM on FI (p < 0.028) and WG (p < 0.036). In the starter and grower phases (1–24 days) and entire cycle (1–35 days), FI did not vary (p > 0.05) between dietary treatments. The FCR was improved (p < 0.023) by CSM in the starter phase mostly in lower and medium CSM diets, and in the grower (p < 0.045) phase, particularly in chickens fed with the maximal level of CSM. Supplementation with Axtra XB enzyme increased WG in the grower (p < 0.037) and finisher (p < 0.036) phases. Enzyme addition significantly improved FCR in the grower (p < 0.008) and finisher (p < 0.017) phases. There was no interaction between enzyme and CSM for gross responses over 1–24 and 1–35 days.

Incorporation of CSM in diets decreased the weight of the small intestine (p < 0.008) and the combined weight of gizzard plus proventriculus (p < 0.048) in starter chicks (Table 5). In grower birds, CSM contributed increased (p < 0.029) the weight of gizzard plus proventriculus with the highest weight recorded in the birds fed medium-CSM diet. The test ingredient decreased (p < 0.013) the weight of the small intestine in growing chickens except for those fed the medium level of CSM. There was no treatment effect (p > 0.05) on the weight of the other visceral organs at 10 or 24 days of age.

There was no interaction between CSM levels and Axtra XB enzyme on the weight of carcass parts. The enzyme supplement significantly increased (p < 0.007) the absolute weight of breast meat (Table 6). Diets containing CSM also resulted in an increase in thigh weight both in absolute (p < 0.024) and relative (p < 0.015) terms. Apart from these carcass parts, there was no treatment effect (p > 0.05) observed in other body parts (meat) yield.

The enzyme increased apparent ileal digestibility of CP (p < 0.030), gross energy (p < 0.014) and starch (p < 0.008) in the ileum (Table 7). However, the apparent ileal starch digestibility was reduced (p < 0.010) by increasing CSM level in the diet, with the lowest starch digestibility noted in chickens fed the diets containing a high level of CSM. There was no effect (p > 0.05) of CSM on CP or gross energy digestibility.

Apart from the CSM–enzyme interaction observed on FI, feed consumption did not differ between the dietary treatments and the control diet. This can be explained by the fact that, although CSM is high in fibre, the experimental diets were formulated in such a way that all nutrients were similar and balanced to meet the birds’ nutrient requirements. This supports the results of a feeding trial conducted by Adeyemo and Longe [20] in which no specific FI pattern in meat chickens fed CSM-containing diets was found, although after the seventh week of their trial, differences between treatments means emerged. The enzyme interacted with CSM on WG response in starting birds and the values of this response were identical between birds fed CSM- and SBM-containing diets both in the growing and finishing phases. CSM can cause growth depression in chicks and this could, among other factors, be due to decreased amino acids digestibility, low lysine content and the presence of gossypol [21]. However, digestibility of amino acids, was improved in the present trial, which can counteract the gossypol effect and growth depression as stated by Henry et al. [22] and Nagalakshmi et al. [23]. These findings agree with the results obtained by Ojewola et al. [2] who reported that the WG of broilers fed CSM-containing diets was similar to that of birds fed SBM diet. However, this was not supported other work which recorded a significant differences in WG when CSM replaced SBM in broiler diets [20]. These authors demonstrated a similarity in gross response values among chickens fed diets in which soybean cake was replaced by cottonseed cake at 75% compared to those fed a control diet. They also stated that a complete replacement of SBM with CSM resulted in depressed growth performance. However, in the present trial, the replacement of SBM with CSM by even up to 90% (18% CSM diet) did not severely affect the gross responses. Enzyme supplementation in grower and finisher diets increased the WG and this may be due to its contribution to the improvement in nutrient digestibility, as previously reported that supplementation wheat-based diets with β-glucanase increased broiler growth [24].

It has been stated that starting chick diets containing high levels of CSM may have a depressing effect on growth coupled with increased feed-to-gain ratios due to the presence of gossypol [2]. Also it was stated that CSM-containing feeds consumed by birds were not converted as effectively as SBM diets due to poor utilisation of amino acids from CSM [20]. However, in the present trial, no statistical difference was observed in finisher chicks because of CSM incorporation in their diets. Most importantly, this proteinaceous ingredient contributed to a reduction in FCR in the starter and grower phases. As mentioned earlier, the enzyme supplement lowered the FCR in grower and finisher birds. These improvements could have resulted from enhanced energy and protein digestibility, initiated by the inclusion of the test enzyme. Feed conversion ratio has been shown to decrease in birds provided with diets containing a β-glucanase and xylanase cocktail [12].

In the grower birds, CSM increased the weight of the gizzard and proventriculus. It is well established that the increased content of fibre in a diet can increase the length and size of the GIT [25,26]. Adding indigestible materials, such as wood shavings, can tremendously increase the weight of gizzard [27]. Other studies have linked the fibre content in dietary ingredients, such as CSM with hypertrophy of the GIT, including the gizzard [28,29]. Although, the fibre content of the formulated diets in this feeding trial was similar to the standard recommended by Aviagen [13], but CSM fibre tends to be physically bulky [30]. In the present trial, the activity of the test enzyme on wheat–sorghum–CSM diets decreased the small intestinal weight, but the effect was not significant. The earlier work of Brenes et al. [31] also found that the weights of visceral organs (liver, pancreas, small intestine and proventriculus) were not affected by wheat or xylanase. Nayefi et al. [29] observed an increased weight of the gizzard and the rest of the GIT in birds fed CSM-containing diets.

The yield of carcass parts and dressing percentage were expressed in relation to unit liveweight (g/kg) and also absolute weight. The outstanding differences between these carcass parts included increased absolute weight of breast meat mediated through enzyme incorporation. The thighs obtained from birds fed CSM diets were also heavier than chickens fed the control diets. The increased weight of carcass parts can be associated with the improved brirds growth, due to increased nutrient digestibility, especially CP and apparent metabolizable energy (AME). The weights of other carcass parts were similar to those of the control group. There are no previous data on the effect of using this kind of enzyme product in diets containing CSM on carcass parts (meat) yield. The eviscerated yield of meat chickens fed CSM-based diets with or without transgenic modification did not differ from the control group fed SBM diets [6].

Batonon-Alavo et al. [32] reported that CSM inclusion in diets resulted in a decrease in apparent ileal energy digestibility, but in this trial, the gross energy digestibility was not affected by CSM. In addition, different studies have reported that phytase and xylanase supplementation improved AME [33,34]. However, a feeding trial conducted by Munyaka et al. [11] revealed that there was no influence of a xylanase and β-glucanase blend on the AME in meat chickens fed with wheat- and corn-based feeds. In the present feeding trial, starch digestibility decreased in birds fed high levels of CSM, with the lowest value observed in the group with the highest level of CSM. On the other hand, enzyme supplementation resulted in increased digestibility of starch. It has been found that incorporating a xylanase and β-glucanase blend in corn- and wheat-based diets resulted in increased starch digestibility, among other attributes [11]. This may be due to the role of β-glucanase and xylanase in reducing digesta viscosity caused by wheat arabinoxylans and pentosans and elimination of nutrient encapsulation of cell walls existing in many dietary ingredients, resulting in nutrient release [11,12,35]. The results from this feeding trial show that the graded substitution of SBM with CSM in broiler diets did not affect CP digestibility. Enzyme supplementation also resulted in increased apparent CP digestibility in the ileum by up to about 2%. However, Mushtaq et al. [4] found no impact of adding a β-glucanase and xylanase cocktail to CSM-containing diets. The findings of the current study were similar to those obtained by Garcia et al. [26] who indicated that incorporating xylanase in wheat-based feeds improved ileal digestibility of dietary nutrients, including CP.