Complete genome sequence of Corynebacterium sp. SCR221107, encoding biosynthesis of vitamin B12 isolated from the rumen fluid of Holstein dairy cows

Baik, Keun Sik; Ramos, Sonny C; Na, Sang Hoon; Kim, Seon Ho; Son, A Rang; Miguel, Michelle; Lee, Sang Suk

doi:10.5187/jast.2023.e74

J Anim Sci Technol

pISSN: 2055-0391, eISSN: 2672-0191

DOI: https://doi.org/10.5187/jast.2023.e74

Article

Complete genome sequence of Corynebacterium sp. SCR221107, encoding biosynthesis of vitamin B12 isolated from the rumen fluid of Holstein dairy cows

Keun Sik Baik¹, Sonny C Ramos¹, Sang Hoon Na¹, Seon Ho Kim¹, A Rang Son¹, Michelle Miguel¹, Sang Suk Lee¹^,^*

Author Information & Copyright ▼

¹Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea.

^*Corresponding Author: Sang Suk Lee, Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, Suncheon 57922, Korea, Republic of. Phone: +82-61-750-3237. E-mail: rumen@scnu.ac.kr.

© Copyright 2023 Korean Society of Animal Science and Technology. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

<italic>Corynebacterium </italic>sp. SCR221107 was isolated from the rumen fluid of healthy male Holstein dairy cows from a research farm at Suncheon, Jeollanam-do, Korea. <italic>Corynebacterium </italic>sp. SCR221107 is a functional probiotic candidate that produces vitamin B<sub>12</sub>. All <italic>Corynebacterium </italic>sp. SCR221107 was sequenced using the PacBio RS II and Illumina HiSeq platforms and assembled de novo. The complete genome sequence of <italic>Corynebacterium </italic>sp. SCR221107 contained one circular chromosome (3,043,024 bp) with a guanine + cytosine (GC) content of 60.1%. Annotation analysis showed the presence of 2,639 protein-coding sequences, 15 rRNA genes, and 57 tRNA genes. Genome analysis found that <italic>Corynebacterium </italic>sp. SCR221107 encodes various genes associated with vitamin B<sub>12</sub> synthesis and transport. The genomic information provided a detailed understanding of <italic>Corynebacterium </italic>sp. SCR221107, suggesting that this isolate may have potential probiotic applications.

Keywords: Corynebacterium sp.; Holstein dairy cow; de novo assembly; whole genome sequencing

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Complete genome sequence of Corynebacterium sp. SCR221107, encoding biosynthesis of vitamin B12 isolated from the rumen fluid of Holstein dairy cows

Abstract